PONE-D-23-25096 Comparative characteristics of oat doubled haploids and oat × maize addition lines: anatomical features of the leaves, chlorophyll a fluorescence and yield parametersPLOS ONE

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PLOS ONE

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https://www.mdpi.com/2077-0472/13/2/243

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2948986/

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Additional Editor Comments:

The authors have to revise the MS according to reviewer1's suggestions so that the MS can be improved

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Manuscript "Comparative characteristics of oat doubled haploids and oat × maize addition lines: anatomical features of the leaves, chlorophyll a fluorescence and yield parameters" is very interesting.

General comments:

Authors evaluated how the presence of maize chromosomes changes the anatomical parameters of the leaves and functioning of photosynthetic apparatus of the disomic OMA lines compared to the DH lines and Bingo cultivar. Authors assessed also yield components for all lines.

Detailed comments:

The introduction is quite interesting. However, most of the references are very old.

Table 2 shows the results of the one-way ANOVA. I suggest supplementing is with mean squares for the difference factor and for the error. Then the table will be more readable.

Table 3: The title says that the table should include SE. Unfortunately, these values are missing from the table. It should be completed.

Table 4 shows the results of the one-way ANOVA. I suggest supplementing is with mean squares for the difference factor and for the error. Then the table will be more readable.

Table 5 shows the results of the one-way ANOVA. I suggest supplementing is with mean squares for the difference factor and for the error. Then the table will be more readable.

Figure 3: The caption says that the drawing should include SE. Unfortunately, these values are missing from the drawing. It should be completed.

Figure 4: The caption says that the drawing should include SE. Unfortunately, these values are missing from the drawing. It should be completed.

The description of statistical methods is cursory. Among other things, information about the empirical distribution and meeting the assumptions of analysis of variance is missing.

My suggestions:

The Authors evaluated yield components for all lines. Unfortunately, they were not tempted to analyze the effect of yield components on yield. This is a very important aspect in breeding. Such an analysis would have increased the value of the manuscript. I suggest conducting such an analysis.

In the Introduction, the authors wrote that, to the best of their knowledge, this is the first time that OMA and DH lines produced from the same parental crosses have been compared in terms of leaf anatomical characteristics. This would suggest a comparison between groups of OMA and DH lines. Unfortunately, such a comparison is missing from the manuscript. I suggest making such a comparison using, for example, contrast analysis.

The manuscript provides a description of many features. Each was discussed separately. In plant breeding, it is very important to determine the relationship between traits. This is very important in the selection process. I suggest conducting a correlation analysis.

Depending on the existence or absence of trait correlation, the selection process in breeding proceeds in different ways. The results of multivariate analysis are undoubtedly facilitating selection decisions. Supplementing the manuscript with the results of the analysis of canonical variables, together with the estimation of Mahalanobis distances between the studied genotypes, will be a valuable enrichment of the conducted research.

Paper needs major revision.

Reviewer #2: Dear Authors,

thank you for a lovely paper that neatly describes the study you have done. I enjoyed reading it. I have made some very minor language edits to the paper which I hope will contribute positively.

The file is attached for your use.

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Reviewer #1: Yes: Jan Bocianowski

Reviewer #2: No

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Attachments

Attachment

Submitted filename: PONE-D-23-25096_reviewer.pdf

Comparative characteristics of oat doubled haploids and oat × maize addition lines: anatomical features of the leaves, chlorophyll a fluorescence and yield parameters

PONE-D-23-25096R1

Dear Dr. Warchol,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Mayank Gururani

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #3: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #3: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #3: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #3: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #3: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The Authors have taken all my comments into account when improving the manuscript. I recommend publishing the manuscript in its current form.

Reviewer #3: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Jan Bocianowski

Reviewer #3: No

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