PONE-D-23-31386Exploration adhesion properties of Liquorilactobacillus and Lentilactobacillus isolated from two different sources of tepache kefir grainsPLOS ONE

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Academic Editor

PLOS ONE

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Reviewer #1: Yes

Reviewer #2: Partly

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Title: Exploration adhesion properties of Liquorilactobacillus and Lentilactobacillus isolated from two different sources of tepache kefir grains

o Reduce the Plagiarism up to 15 %

Abstract:

o The results are not efficiently summarized in the abstract. Write 2 line of research scope at start.

Introduction:

� Mention at least some of the latest references related to Lentilactobacillus

� The introduction is over generalized. Improve it add relevant references.

� Anti-bacterial activity of essential oils against multidrug resistant foodborne pathogens isolated from raw milk

� Isolation and Characterization of a Cholesterol-Lowering Bacteria from Bubalus bubalis Raw Milk

� Antagonistic, Anti-oxidant, Anti-inflammatory and Anti-diabetic Probiotic Potential of Lactobacillus agilis Isolated From the Rhizosphere of the Medicinal Plants

� Recent Innovations in Non-dairy Prebiotics and Probiotics: Physiological Potential, Applications, and Characterization

Materials and Methods:

� Italicize the names of microorganisms.

� Add sample collection perform as supplementary data

� Line 103, Staphylococcus aureus (ATCC 25923), write subspecies as well with each pathogen

� Line 259; containing 5% (w/v) human blood, or sheep bold? Cross check

� Line 318, MRS v

Discussion:

Please add some latest references and improve the discussion further.

o Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics

o Biosurfactant Screening and Antibiotic Analysis of Bacillus salmalaya

o Enhancing Lipase Production of Bacillus salmalaya Strain 139SI Using Different Carbon Sources and Surfactants

Seems over generalized the discussion part, kindly improve

References

Double check the references according to the Journal style.

Reviewer #2: The authors have analyzed the adhesive properties of Liquorilactobacillus and Lentilactobacillus isolated

from two different sources of tepache kefir grains in vitro which is a very basic study. In vitro adhesion properties may or may not replicate in vivo and for the development of probiotics or beneficial microbes, it is important that authors study the adhesion properties in appropriate cell lines and also in vivo in appropriate animals studies.

This study have reported only very basic in vitro characterization of few strains and to add the credibility and to understand the true probiotic potential authors should do in vivo studies as well.

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Reviewer #1: No

Reviewer #2: No

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Exploration adhesion properties of Liquorilactobacillus and Lentilactobacillus isolated from two different sources of tepache kefir grains

PONE-D-23-31386R1

Dear Dr. Castro-Rodríguez,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Guadalupe Virginia Nevárez-Moorillón, Ph.D.

Academic Editor

PLOS ONE

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