PONE-D-23-38791Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MSPLOS ONE

Dear Dr. Zhang,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

==============================The submitted manuscript titled "Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MS"  can be considered for publication in "Plos One" journal with minor revision. Authors require some minor modification in submitted manuscript as suggested by the learned reviewers.  Reviewer's comments are as follows:

Reviewer #1: Finding of the reviewed manuscript will give new source of information to the scientific community. It is relevant work and also good effort from the author's side. So the manuscript may be accepted for possible publication in the Plos One after considering all the formalities and rules and regulations of the Plos One publication policy as well overall publication guidelines.

Reviewer #2: In this manuscript, authors studied the “Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MS”. It is a good piece of research that will work for understanding about embryonic development. Authors have studied about the identification of metabolites and metabolite changes in egg yolk that provides nutrient requirement in goose embryonic development. In this review, authors have summarized that may provide new ideas for improving prehatch embryonic health and nutrition.

Authors should ensure that they have followed the Instructions to Authors of this journal. Overall, the manuscript in its current form is not acceptable for publication in the esteemed “Plos One” journal. Authors require minor revisions in the manuscript and can submit the revised manuscript. Some important comments listed as follows:

1. On Page 2, Line 26, please correct E (7) and define the numbers.

2. In abstract, authors have written that yolk metabolites were approximately similar at E7 and E12, E23 and E28 but what about E18.

3. Abbreviations used first time in the text should be explanatory (Page 3, Line 56).

4. The format of heading should be uniform throughout manuscript.

5. Please cite the references form where extraction procedure and other methodologies were adopted.

=============================

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We look forward to receiving your revised manuscript.

Kind regards,

Pankaj Singh, Ph.D.

Academic Editor

PLOS ONE

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"This work was supported by two grants received from the Science and Technology Project of Guizhou Province, China (No. QKHZC[2021]YB156 and No.QKHZC[2020]1Y047)."

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Finding of the reviewed manuscript will give new source of information to the scientific community. It is relevant work and also good effort from the author's side. So the manuscript may be accepted for possible publication in the Plos One after considering all the formalities and rules and regulations of the Plos One publication policy as well overall publication guidelines.

Reviewer #2: In this manuscript, authors studied the “Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MS”. It is a good piece of research that will work for understanding about embryonic development. Authors have studied about the identification of metabolites and metabolite changes in egg yolk that provides nutrient requirement in goose embryonic development. In this review, authors have summarized that may provide new ideas for improving prehatch embryonic health and nutrition.

Authors should ensure that they have followed the Instructions to Authors of this journal. Overall, the manuscript in its current form is not acceptable for publication in the esteemed “Plos One” journal. Authors require minor revisions in the manuscript and can submit the revised manuscript. Some important comments listed as follows:

1. On Page 2, Line 26, please correct E (7) and define the numbers.

2. In abstract, authors have written that yolk metabolites were approximately similar at E7 and E12, E23 and E28 but what about E18.

3. Abbreviations used first time in the text should be explanatory (Page 3, Line 56).

4. The format of heading should be uniform throughout manuscript.

5. Please cite the references form where extraction procedure and other methodologies were adopted.

**********

6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

**********

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Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MS

PONE-D-23-38791R1

Dear Dr. Zhang,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org.

If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Pankaj Singh, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: The revised manuscript entitled “Metabolomics analysis of the yolk of Zhijin white goose during the embryogenesis based on LC-MS/MS” submitted for publication can be accepted for publication in esteemed "Plos One" journal. Authors have addressed each queries very well raised by reviewer and have critically modify the manuscript as per requirement.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #2: No

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