PONE-D-23-37831Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteriaPLOS ONE

Dear Dr. Ouandaogo,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Partly

Reviewer #4: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: N/A

Reviewer #4: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: No

Reviewer #4: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: No

Reviewer #4: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Reviewer’s Constructive Feedback to the Authors:

I have had the opportunity to review your study titled "Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteria." The abstract provides a clear overview of the study, which focuses on the use of Ocimum kilimandscharicum for the biological production of silver nanoparticles (AgNPs) and their potential antibacterial properties. There is no specific fault in the given text. However, here are some comments and suggestions:

1. There is inconsistency in the use of tenses. For example, the phrase "is becoming increasingly important" is in the present continuous tense, while the rest of the abstract is predominantly in the past tense.

2. A few typos in the document, such as aluminium instead of aluminum, space problem, comma and fullstop issues. Additionally, there are some other grammatical errors that may need attention.

3. The results and discussion sections are written together in this manuscript. I don't know if it falls within the rules of the journal or not. It is preferable to place this in different parts.

4. Picture quality should be increased, especially for Fig. 19a, b; For MIC and MBC they can include the picture of broth culture.

In this study the authors focuses on the synthesis of silver nanoparticles (AgNPs) using aqueous and methanolic extracts of Ocimum kilimandscharicum leaves. The UV-Vis analysis confirmed the presence of silver nanoparticles through the observation of their surface plasmon resonance. The biosynthesized AgNPs exhibited significant antibacterial activity against various pathogens. Considering the growing concerns regarding microbial resistance, AgNPs hold great potential as antimicrobial agents. Further research is recommended to investigate the in vitro and in vivo toxicity of these sustainable silver nanoparticles

Reviewer #2: The author has written a manuscript titled Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteria.

1) manusript came out well

2) In introduction, add some more points and literature review

3) In fugure 2 Menthanol crude extrat graph is above the range, do it once again and add in the graph

4) You have added plain silver meance nano or silver nitrate; please express correctly

5) Figure 3 only writes an OD in nm not an absorbance.

6) You have performed the experiment with aquous extract and methanol extract. need comparative study on all phytochemical analyses and which one is best to isolate bioactive molecules

7) In figure 6, why is there is peak in 360nm and what is the significance?

8) please name the peaks

9) Figure 8 and 9 what is a comparison interpretation?add

10) only one application is done; somemore more applications like anti-cancer activity, environmental study , antioxidant activity etc

Reviewer #3: Dear editor and author

Overall this is a good article that presents a novel synthesis strategy and good characterization techniques. However, I would like to make some suggestions.

I am attaching the manuscript with some comments I would like you to address.

1.There are lots of typo and grammatical mistakes in the manuscript making the manuscript less interesting to read.

2.IN FTIR studies, these functional groups indicated flavonoids, phenols, and ascorbic acid in Ocimum kilimandscharicum leaf extract-----FTIR can indicate only functional groups and not presence of compounds or metabolites.

3.XRD and EDX, and TEM added the manuscript.

4.The novelty of the research work in terms of prior art available has to be brought out.

Reviewer #4: The authors did interesting research work with a lot of results. thus, suggestion to combine or choose either figures or tables for some results. for example, FT-IR spectra are not needed when the table resumes all the results for it.

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Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Shahina Akter, Principal Scientific Officer, Bangladesh Council of Scientific and Industrial Research (BCSIR), Email- shupty2010@gmail.com

Reviewer #2: No

Reviewer #3: No

Reviewer #4: Yes: Amadou Issoufou

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Attachments

Attachment

Submitted filename: Reviewer-comments to the author-Plos one-1.docx

Attachment

Submitted filename: Dear editor and author.docx

Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteria

PONE-D-23-37831R1

Dear Dr. Ouandaogo,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Jorddy Neves Cruz

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Reviewer’s Constructive Feedback to the Authors:

The revision of the manuscript titled "Silver nanoparticle biosynthesis utilizing Ocimum kilimandscharicum leaf extract and assessment of its antibacterial activity against certain chosen bacteria" reflects a commendable effort by the authors to address all queries raised during the initial review process. The manuscript has undergone significant improvements in clarity, methodology, and data presentation. The authors have successfully elucidated the significance of their research objectives and provided comprehensive details regarding the biosynthesis of silver nanoparticles using Ocimum kilimandscharicum leaf extract. Methodological enhancements, including the incorporation of controls and standardization techniques, have strengthened the experimental approach. Moreover, the presentation of experimental data through well-organized tables and figures, along with statistical analyses, enhances the reliability of the reported results. Minor revisions, such as adjustments to referencing style and language refinement, have also been effectively addressed. Based on the thorough revisions made, I recommend acceptance of the manuscript for publication, pending final approval from the editorial board.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Dr. Shahina Akter

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Attachments

Attachment

Submitted filename: 1-Reviewer-comments to the author.docx