PONE-D-23-23404Transcriptome and metabolomics analysis of adaptive mechanism of Chinese mitten crab (Eriocheir sinensis) to aflatoxin B1PLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: N/A

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Grammatical and spelling mistakes

Alignment not proper

Scientific names should be in italics

Please change ul-µl throughout the manuscript

Comparison data, results, or references should be there in the Results section

Statistical data should be explained more properly

Line 48- Food and feed?

Line 58 and 59- Rewrite the sentence

Line 81- We hope? It is research work and if results are there then why expect?

Line 96 and Line 101- “certain amount”- Please mention the amount

Line 107-Line 109- After 30 minutes or 60 minutes? Give only one-time point

that was done for the experiment

Line111- “Frozen samples with liquid nitrogen”- Rewrite the sentence

Line 126- Different groups- Mention the groups

Line 139-Line 141-Rewrite the sentence-It is not clear

Line 149-Line 150- Rewrite the sentence-Not clear

Line 170-Line 173- Explain the statistical analysis in detail

Line 191- PC1 and PC2- What are these? Explain?

Line 201- Line 205- Break the sentences to make it more clear

Reviewer #2: The manuscript entitled “Transcriptome and metabolomics analysis of the adaptive mechanism of Chinese mitten crab (Eriocheir sinensis) to aflatoxin B1” is an interesting study and the authors have collected a unique dataset using a cutting-edge methodology. The paper is generally well-written and structured and is presented in an intelligible fashion written in Standard English. This is described in a technically sound piece of scientific research with data that supports the conclusions. Experiments were conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions are appropriately based on the data presented in an appropriate and rigorous statistical analysis.

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: Manuscript SSE3092023.docx

Transcriptome and metabolomics analysis of adaptive mechanism of Chinese mitten crab (Eriocheir sinensis) to aflatoxin B1

PONE-D-23-23404R1

Dear Dr. Xiaohua Zhu 

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Amel Mohamed El Asely

Academic Editor

PLOS ONE