PONE-D-23-18792Soil fauna-microbial interactions complexity triggers shifts in microbial communities under a contamination disturbance.PLOS ONE

Dear Dr. Yergeau,

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PLOS ONE

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"This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant RGPIN-2014-05274 and strategic grant for projects STPGP 494702) to E.Y

S.C.G. was supported by the Research Affiliate Program from the Government of Canada. V.C. and J.A.D. were both supported by the Undergraduate Student Research Awards from the Natural Sciences and Engineering Research Council of Canada. E.M.K. was supported by a scholarship from the Armand-Frappier Foundation. This research was enabled in part by support provided by Calcul Québec (www.calculquebec.ca) and Compute Canada. "

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"This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant RGPIN-2014-05274 and strategic grant for projects STPGP 494702) to E.Y S.C.G. was supported by the Research Affiliate Program from the Government of Canada. V.C. and J.A.D. were both supported by the Undergraduate Student Research Awards from the Natural Sciences and Engineering Research Council of Canada. E.M.K. was supported by a scholarship from the Armand-Frappier Foundation. This research was enabled in part by support provided by Calcul Québec (www.calculquebec.ca) and Compute Canada."

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"This work was supported by the Natural Sciences and Engineering Research Council of Canada (Discovery grant RGPIN-2014-05274 and strategic grant for projects STPGP 494702) to E.Y

S.C.G. was supported by the Research Affiliate Program from the Government of Canada. V.C. and J.A.D. were both supported by the Undergraduate Student Research Awards from the Natural Sciences and Engineering Research Council of Canada. E.M.K. was supported by a scholarship from the Armand-Frappier Foundation. This research was enabled in part by support provided by Calcul Québec (www.calculquebec.ca) and Compute Canada."

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: Author are recommended to do needful

1.Introduction must be expanded

2.future prospective must be strengthen

3.An abstract figure or work flow must be prepared

4.RT PCR and Illumina Seq work are remarkable and highly appreciable

Reviewer #2: Dear Authors, The manuscript has been written very well and the research plan is very scientific and sound. The MS has shown the original findings and contradictory results with concerned previous studies. It is always good to show the all aspects of research findings either positive or negative.

However, it is suggested to revise the title of the manuscript as per finding if possible.

Line number 76, potting mix is okay or potting mixture?

Kindly rewrite the sentence , line number 188-189.

Use brackets to before writing supplementary table name and number in the MS. ex. for line number 201-202

Kindly check for all the abbreviations used that must be in full form at least ones in the MS.

The abstract section will be better if avoid too much abbreviations.

The conclusion section is too crispy. Therefore, authors can elaborate it in few more lines.

The community composition section in the results has been written vastly, authors can reduce these lines by citing concerned tables to increase the readability. For examples, all the species name is not essential to write, it can be referred to the tables.

The data analysis and statistical analysis part have been performed very well.

The MS can be accepted in this form after suggested few minor corrections.

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Reviewer #1: Yes: Dr Kuldip Jayaswall

Reviewer #2: Yes: Deepanshu Jayaswal

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Soil fauna-microbial interactions shifts fungal and bacterial communities under a contamination disturbance.

PONE-D-23-18792R1

Dear Dr. Yergeau

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Tunira Bhadauria, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments: