PONE-D-22-35213Multifactorial assessment of leukocyte reduced platelet rich plasma injection in dogs undergoing tibial plateau leveling osteotomy: a retrospective studyPLOS ONE

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #2: Yes

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Reviewer #1: The paper is very interesting and well written.

As a work on PRP, the authors may consider including data on the number of platelets in the blood of patients before surgery, which, with the centrifugation method used, would allow for the standardization of the PRP used.

Reviewer #2: This manuscript examined the effects of leukocyte reduced platelet rich plasma (lPRP) on surgical site infection (SSI), implant removal, bone healing, clinical signs, progression of OA in the dogs undergoing tibial plateau leveling osteotomy (TPLO). As the results, lPRP treatment significantly improved bone healing, global OA scores, and lameness score. However, there were no significant differences in the results of SSI and implant removal rate. The content of this paper is worthy of publication in Plos One, because of its contribution to the clinical field of veterinary medicine. However, some revisions of manuscript are needed before it can be accepted for publication.

Introduction (line 79): “1PRP” is a first appearance. Please list the term that is not abbreviations (Ex. Leukoreduced platelet rich plasma?).

M&M (line 87-93, 136-137): How did you divide the subject dogs into two groups? What were the criteria of surgeon's preference?

M&M (line 101-103): Orthopedic (excluding CrCL rupture) and neurological disorders that may affect gait and mobility should be also added to the exclusion criteria.

M&M (line 139-142): This content is described below (lines 164-167), so delete the relevant text.

M&M (line 150): pet→dog

M&M (line 151): Please describe which type of antibiotic was administered orally.

Results (line 262-270): Did you compare post-op TPA between groups? These values might affecte the outcome of the surgery.

Results (line 277-281): Add the sentence about significant difference in the presence of synovitis between groups.

Table 2: Correct any misalignments in the Table 2 (Incision appearance at suture removal).

Discussion (line 415-425): In this paragraph, you need to add a discussion of the role of PRPs in infection protection and the results of this study.

Discussion (line 438-442): leukocyte reduced PRP→lPRP?

Discussion: In this study, lameness was significantly improved in the lPRP group. You should discuss whether the accelerated bone healing in the lPRP group was the result of improved loading on the tibial bone or the effect of PRP in the Discussion.

Reference: There are two No. 29 references. Please check again, including the citation in the text.

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Reviewer #1: No

Reviewer #2: Yes: Kazuya Edamura

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Multifactorial assessment of leukocyte reduced platelet rich plasma injection in dogs undergoing tibial plateau leveling osteotomy: a retrospective study

PONE-D-22-35213R1

Dear Dr. Nicole Buote

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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