Expression, activity, and consequences of biochemical inhibition of α- and β-glucosidases in different life stages of Culex quinquefasciatus

Edwin R. Burgess IV; Neil D. Sanscrainte; Caitlin E. Taylor; Lyle J. Buss; Alden S. Estep

doi:10.1371/journal.pone.0286609

Peer Review History

Original SubmissionMay 18, 2023
24 Jul 2023 Decision Letter - Rachid Bouharroud, Editor PONE-D-23-15295Expression, activity, and consequences of biochemical inhibition of α- and β-glucosidases in different life stages of Culex quinquefasciatusPLOS ONE Dear Dr. Burgess, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Please submit your revised manuscript by Sep 07 2023 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 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If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice. Additional Editor Comments: Dear Authors The work deserve to be published but some minor comments should be addressed in order to achieve best results. Regards [Note: HTML markup is below. Please do not edit.] Reviewers' comments: Reviewer's Responses to Questions Comments to the Author 1. Is the manuscript technically sound, and do the data support the conclusions? The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented. Reviewer #1: Yes Reviewer #2: Yes ******** 2. Has the statistical analysis been performed appropriately and rigorously? Reviewer #1: Yes Reviewer #2: Yes ****** 3. Have the authors made all data underlying the findings in their manuscript fully available? The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified. Reviewer #1: Yes Reviewer #2: Yes ****** 4. Is the manuscript presented in an intelligible fashion and written in standard English? PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here. Reviewer #1: Yes Reviewer #2: No ****** 5. Review Comments to the Author Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) Reviewer #1: The results reported by this study are original and have not been published elsewhere and suggest the importance of α- and β-glucosidases on the survival in the 3 life stages of Cx. Quinquefasciatus studied. The experiments were described with sufficient details and the statistical analysis were well performed. The conclusions were supported accordingly to the data; however, the discussion may need a fresh literature for more clarity about the research in parallel that were done during the last few years. Therefore, I recommend a minor correction. Reviewer #2: The manuscript is interesting and provides valuable insights into the role of α-glucosidases and β-glucosidases in the survival of Cx. quinquefasciatus mosquitoes in different life stages. The findings contribute to our understanding of mosquito digestion, species-specific knowledge, biocontrol agents, and potential applications for vector control strategies. However, I suggested an English review, I added some comments in the pdf. file. ****** 6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files. If you choose “no”, your identity will remain anonymous but your review may still be made public. Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy. Reviewer #1: No Reviewer #2: Yes: Maísa da Silva Araújo ******** [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. Attachments Attachment Submitted filename: PONE-D-23-15295_MA20230717.pdf https://doi.org/10.1371/journal.pone.0286609.r001
Revision 1
31 Jul 2023 Author Response Response to reviewers: We thank the reviewers for their helpful comments that have improved our manuscript. We have made comments below addressing the general review statement. Additional, Reviewer #2 sent along a document with grammatical comments. We have generated a Word document with Track Changes added to it documenting which changes we made and which we didn’t, along with an explanation for why we chose not to make the change. Comments below are preceded by *. Reviewer #1: The results reported by this study are original and have not been published elsewhere and suggest the importance of α- and β-glucosidases on the survival in the 3 life stages of Cx. Quinquefasciatus studied. The experiments were described with sufficient details and the statistical analysis were well performed. The conclusions were supported accordingly to the data; however, the discussion may need a fresh literature for more clarity about the research in parallel that were done during the last few years. Therefore, I recommend a minor correction. We thank this reviewer for the flattering words on our work. We were very unsure of what this reviewer means by “fresh literature” about the “research in parallel.” To our knowledge, and with extensive literature searches preceding the submission of this manuscript, we found very little recent work done on the topic of glucosidases in mosquitoes. Of the ones we found, we addressed them in the introduction. As it stands, we feel that the discussion addresses our discoveries based on what is known and accepted in the entomological literature on this topic. We will default to what the editor would like us to do, if anything, to address this comment. Reviewer #2: The manuscript is interesting and provides valuable insights into the role of α-glucosidases and β-glucosidases in the survival of Cx. quinquefasciatus mosquitoes in different life stages. The findings contribute to our understanding of mosquito digestion, species-specific knowledge, biocontrol agents, and potential applications for vector control strategies. However, I suggested an English review, I added some comments in the pdf. file. *This reviewer provided a comprehensive review of our document’s grammar and syntax. We thank them for catching some errors and in general striving to improve the readability and flow of our manuscript. We have made a good number of the requested changes, while leaving others unchanged. We have added our changes and response to the comments in a Track Changes version of our manuscript. Attachments Attachment Submitted filename: Response to Reviewers.docx* https://doi.org/10.1371/journal.pone.0286609.r002
9 Aug 2023 Decision Letter - Rachid Bouharroud, Editor Expression, activity, and consequences of biochemical inhibition of α- and β-glucosidases in different life stages of Culex quinquefasciatus PONE-D-23-15295R1 Dear Dr. Burgess, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Rachid Bouharroud Academic Editor PLOS ONE Additional Editor Comments (optional): Dear Happy for the acceptance of this valuable work related to public health. Your manuscript looks currently good to be published by PlosOne. Regards Reviewers' comments: https://doi.org/10.1371/journal.pone.0286609.r003
Formally Accepted
21 Aug 2023 Acceptance Letter - Rachid Bouharroud, Editor PONE-D-23-15295R1 Expression, activity, and consequences of biochemical inhibition of a- and b-glucosidases in different life stages of Culex quinquefasciatus Dear Dr. Burgess: I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. If we can help with anything else, please email us at plosone@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Rachid Bouharroud Academic Editor PLOS ONE https://doi.org/10.1371/journal.pone.0286609.r004

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