PONE-D-23-04874Comparative study on chloroplast genomes of three Hansenia forbesii varieties (Apiaceae)PLOS ONE

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https://www.frontiersin.org/articles/10.3389/fpls.2022.1099856/full

https://www.sciencedirect.com/science/article/pii/S0888754319308420?via%3Dihub

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Additional Editor Comments:

The manuscript is well written. Comparative analysis needs to be more detailed in terms of number of polymorphic loci (SNP, indels, deletion and duplication). The authors should report indels and large deletions and make an effort to design primers capturing deletions, indels and hypervariable regions, so that polymorphic markers can be developed for varietal discrimination. Discussion needs to be more precise in relation to functional changes in proteins.

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: Discussion and Conclusion section I would like to request more precisely indicate actual fact compare with others findings and real application in the field of life science .

Discussion and Conclusion section I would like to request more precisely indicate actual fact compare with others findings and real application in the field of life science .

Reviewer #2: This is an interesting manuscript by Chenghao Zhu et al. elucidate the chloroplast genome of Hansenia forbesii varieties. The manuscript is well written and organized. Alike the interesting manuscript, I have found some minor issues that should be considered in your manuscript in future. Hence, the authors should take into consideration the following points in order to improve the manuscript.

1. Detailed descriptions of the chloroplast genome's usual properties should be contained in a single paragraph in the introduction. Moreover, the necessity of exploring the chloroplast genome in Hansenia forbesii is not clearly presented also.

2. A supplementary table showing the basic information of previously decoded chloroplast genome related to Hansenia species and model plant

3. Lines 134–136 gene type counting error.

4. In line 148-150 we know 3bp create a code, then in protein sequence one more or one less bp how create a three letter code.

5. Table 1: There is no statistical analysis in presenting the quantitative parameters.

6. How did you separate chloroplast DNA from the nuclear DNA?

7. How many biological replications were considered during library preparation for sequencing?

8. Line 93-94: National Center of Biotechnology Information. It must be National Center for Biotechnology Information.

9. The authors failed to interpret the results of three chloroplast genome differences, which resulted no molecular description of any morphological differences among the three Hansenia forbesii varieties.

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Reviewer #1: No

Reviewer #2: Yes: Borhan Ahmed

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Comparative study on chloroplast genomes of three Hansenia forbesii varieties (Apiaceae)

PONE-D-23-04874R1

Dear Dr. Zhirong,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Shyamal Krishna Talukder, Ph.D.

Academic Editor

PLOS ONE