PONE-D-23-03391Plasmodium falciparum-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of soluble factors reducing brain microvascular barrier functionPLOS ONE

Dear Dr. Storm,

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Comments to the Author

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: The authors set out “to investigate Plasmodium falciparum-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of soluble factors reducing brain microvascular barrier function”. They co-cultured IT4var14 IE and the monocyte cell line THP-1 for 24 hours and determined whether generated soluble molecules affect barrier function of human brain microvascular endothelial cells. They found that the medium produced after co-culture did not affect endothelial barrier function and similarly no effect was measured after inducing oxidative stress. Anyway, the manuscript can be improved in the following ways:

ABSTRACT

It is better, if it includes the study period,data entry, data collection tool and data analysis methods

Itroduction

Line 35: Plasmodium falciparum (P. falciparum), then use the abbreviated one throughout the whole document.

Line 41: Nk cells (please use the long fom at the first time), then use the abrivated one throughout the whole document.

Line 49: DNA(please write in long form at the 1st time)

Please do the rest in the same way

METHODS

It is better, if it includes the study period, data collection tool and study area (Where??? culture done)

Please, put the materials and methods after the introduction section not after the discussion section

RESULTS: It is ok

DISCUSSION: It is ok

SUMMARY: The article is an original and it gives an important clue. But, It needs minor revision.

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Reviewer #1: No

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Attachments

Attachment

Submitted filename: Review Reports by Abebe Fenta.docx

Plasmodium falciparum-infected erythrocyte co-culture with the monocyte cell line THP-1 does not trigger production of soluble factors reducing brain microvascular barrier function

PONE-D-23-03391R1

Dear Dr. Storm,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Gebreselassie Demeke

Academic Editor

Additional Editor Comments (optional):

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