Commensal Neisseria species share immune suppressive mechanisms with Neisseria gonorrhoeae

Weiyan Zhu; Maria X. Cardenas-Alvarez; Joshua Tomberg; Marguerite B. Little; Joseph A. Duncan; Robert A. Nicholas

doi:10.1371/journal.pone.0284062

Peer Review History

Original SubmissionFebruary 10, 2023
10 Feb 2023 Author Response Attachments Attachment Submitted filename: Response to Review.docx https://doi.org/10.1371/journal.pone.0284062.r001
8 Mar 2023 Decision Letter - Fiona J Radcliff, Editor PONE-D-23-03331Commensal Neisseria species share immune suppressive mechanisms with Neisseria gonorrhoeaePLOS ONE Dear Dr. Nicholas, Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process. Thank you for re-submitting your manuscript. The manuscript, the original reviewer's comments and the minor changes made to the manuscript in response to their reviews have been evaluated. I agree with the original decision that this is an important and interesting piece of work which is of interest to the field. During the re-review process a few minor revisions were identified that are required to clarify the methods used and to correct a statement made in the manuscript's discussion. 1. Methods (line 152-153): were the DCs pulsed with OVA before combination with OT-II CD4+ T cells? Indicate source of OVA, concentration used/incubation time. 2. Methods (line 177-178): What ratio of DC:CD4+ responder cells was used for co-culture assays? 3. Line 323: this sentence requires modification as it implies that reference number 37 includes a Gonorrhoea challenge study. Please submit your revised manuscript by Apr 22 2023 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file. 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The PLOS ONE style templates can be found at https://journals.plos.org/plosone/s/file?id=wjVg/PLOSOne_formatting_sample_main_body.pdf and https://journals.plos.org/plosone/s/file?id=ba62/PLOSOne_formatting_sample_title_authors_affiliations.pdf 2. To comply with PLOS ONE submissions requirements, in your Methods section, please provide additional information regarding the experiments involving animals and ensure you have included details on (1) methods of sacrifice, (2) methods of anesthesia and/or analgesia, and (3) efforts to alleviate suffering. 3. Please include captions for your Supporting Information files at the end of your manuscript, and update any in-text citations to match accordingly. Please see our Supporting Information guidelines for more information: http://journals.plos.org/plosone/s/supporting-information. 4. Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice. Additional Editor Comments: Apologies that this feedback has taken a while to reach you. It took a little while to identify the best way forward with this manuscript. It's an unusual situation given it had already been through the system and received positive reviews. It was unable to go back to the original editor. [Note: HTML markup is below. Please do not edit.] [NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.] While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step. https://doi.org/10.1371/journal.pone.0284062.r002
Revision 1
14 Mar 2023 Author Response Dear Dr. Radcliff: Thank you for your email on our submission [PONE-D-23-03331], with an editorial decision of minor revision. We have edited the manuscript to address the comments raised in the reviews, and our changes are shown below for each point raised. Point 1: � Methods (line 152-153): were the DCs pulsed with OVA before combination with OT-II CD4+ T cells? Indicate source of OVA, concentration used/incubation time. o We agree that the methods as previously written were not clear on these points. The DCs were pulsed for 24 hr with OVA in the presence of either live bacteria, conditioned medium, or purified PorB proteins. The cells were then washed to remove these and then incubated with the OT-II CD4+ T cells for 7 days. We have clarified this point in the revised manuscript and included all the requested information. Point 2: � Methods (line 177-178): What ratio of DC:CD4+ responder cells was used for co-culture assays? o The ratio of DC:CD4+ responder cells was 1:10 in the co-culture assays. We have included additional information to clarify the methods used. Point 3: � Line 323: this sentence requires modification as it implies that reference number 37 includes a Gonorrhoea challenge study. o We thank the reviewer for catching this mis-statement; the study indeed did not include an Ng challenge study, and instead was looking at the cross-reactive bactericidal Abs produced by immunized mice. We have modified the original sentence to reflect this. I am also including our response to a subsequent email to address two additional points before the article will be considered for publication. The points of the email are below, along with our responses. � To comply with PLOS ONE submissions requirements, in your Methods section, please provide additional information regarding the experiments involving animals and ensure you have included details on (1) methods of sacrifice, (2) methods of anesthesia and/or analgesia, and (3) efforts to alleviate suffering. o We have included additional information regarding the points above in the Ethics statement at the beginning of the Methods section. � 2. Please note that PLOS ONE requires submissions reporting blot or gel data to comply in full with the reporting requirements described at https://journals.plos.org/plosone/s/figures#loc-blot-and-gel-reporting-requirements. We now require authors to provide the original unadjusted and uncropped images for any blot or gel data reported in PLOS ONE submissions. In our internal checks for your submission, we noted that you did not provide original raw image files supporting blot/gel data in response to our previous request. o Please note that we already provided the requested raw images in the Supplemental Information. However, it was apparently not named as requested (S1_raw_images). Therefore, we remade the document in Adobe Illustrator and renamed it S1_raw_images.pdf. The revised manuscript (both tracked and clean) containing all of the above changes have now been uploaded. I have also removed Supplemental Information (since that file is now redundant with the new file S1_raw_images.pdf) and uploaded S1_raw_images.pdf in its place. Attachments Attachment Submitted filename: Response to Review_v2.docx https://doi.org/10.1371/journal.pone.0284062.r003
22 Mar 2023 Decision Letter - Fiona J Radcliff, Editor Commensal Neisseria species share immune suppressive mechanisms with Neisseria gonorrhoeae PONE-D-23-03331R1 Dear Dr. Nicholas, We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements. Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication. An invoice for payment will follow shortly after the formal acceptance. To ensure an efficient process, please log into Editorial Manager at http://www.editorialmanager.com/pone/, click the 'Update My Information' link at the top of the page, and double check that your user information is up-to-date. If you have any billing related questions, please contact our Author Billing department directly at authorbilling@plos.org. If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org. Kind regards, Fiona J Radcliff Academic Editor PLOS ONE Additional Editor Comments (optional): Reviewers' comments: https://doi.org/10.1371/journal.pone.0284062.r004
Formally Accepted
30 Mar 2023 Acceptance Letter - Fiona J Radcliff, Editor PONE-D-23-03331R1 Commensal Neisseria species share immune suppressive mechanisms with Neisseria gonorrhoeae Dear Dr. Nicholas: I'm pleased to inform you that your manuscript has been deemed suitable for publication in PLOS ONE. Congratulations! Your manuscript is now with our production department. If your institution or institutions have a press office, please let them know about your upcoming paper now to help maximize its impact. If they'll be preparing press materials, please inform our press team within the next 48 hours. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information please contact onepress@plos.org. If we can help with anything else, please email us at plosone@plos.org. Thank you for submitting your work to PLOS ONE and supporting open access. Kind regards, PLOS ONE Editorial Office Staff on behalf of Dr. Fiona J Radcliff Academic Editor PLOS ONE https://doi.org/10.1371/journal.pone.0284062.r005

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