PONE-D-22-30432Antibacterial Potential of Luidia clathrate (Sea Star) Tissue Extracts Against Selected Pathogenic BacteriaPLOS ONE

Dear Dr. Mustafa,

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PLOS ONE

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Additional Editor Comments:

The reviewers have serious concerns about the novelry and property of this work. The Ms should be revised carefully following the reviewers comments.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

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Reviewer #1: No

Reviewer #2: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: No

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Reviewer #1: No

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: * The manuscript is poorly written. It has plenty of grammatical errors as well as sentence-structure mistakes.

* The paper does not make a significant contribution to new knowledge in the discipline.

* The research idea lacks novelty.

Reviewer #2: Abstract: It is not clear which pathogens are tested in the abstract (line 35). It is worth mentioning the species names. In the methodological part of the abstract, the authors should specify which organs of sea star were used for the experiments. The abstract should clearly present the scope of the work and its effects.

Material and methods: How many organisms were purchased for research (line 99)? How long they were kept in the lab before the analysis (line 101-102)? Line 104-105: Which organs were taken for testing. It's not clear from these two lines: only body wall and gonad - or any other components too (gut)? It should be clear what type of materials were used for the investigation. Line 111: the purity and concentration of the extractants used and their manufacturer/supplier were not specified. Line 112: shaker – the mark and model should be added. Line 114: how long the decantation was carried out. Line 116: what was the pressure applied? Line 124-127: what was the origin of the bacteria used in the research, how they were propagated for the research? To determine the antibacterial activity of the extracts, the authors should perform chemical analyzes of these extracts. This data should be included in to research article. In the current state the results presented in the paper are too poor to be published.

Taking into account the above deficiencies in the description of the methodology, further review proceedings can be conducted after their completion.

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Reviewer #1: No

Reviewer #2: No

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Antibacterial Potential of Luidia clathrata (Sea Star) Tissue Extracts Against Selected Pathogenic Bacteria

PONE-D-22-30432R1

Dear Dr. Mustafa,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Estibaliz Sansinenea

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

The authors have followed all recommendations closely improving their MS, they have changed the abstract and made the pertinent clarifications, therefore the MS can be accepted in the current form.

Reviewers' comments: