PONE-D-22-26011Oncometabolic Role of Mitochondrial Sirtuins in Glioma Patients.PLOS ONE

Dear Dr. Mahjabeen,

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https://www.futuremedicine.com/doi/10.2217/fon-2021-0264

https://www.mdpi.com/2072-6694/9/7/90/htm

https://www.futuremedicine.com/doi/10.2217/fon-2019-0365

https://www.frontiersin.org/articles/10.3389/fonc.2018.00622/full

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Comments to Authors:

This is an interesting work PONE-D-22-26011 Oncometabolic Role of Mitochondrial Sirtuins in Glioma Patients. which aims to clarify the regulation of mitochondrial sirtuins and related genes in glioma cells. This work lays the foundation for future research in the study of gliomas. To improve your manuscript, some suggestions are listed below:

1) The abstract is quite long and you may risk losing the reader’s attention. I suggest you rewrite it in a more synthetic way in order to just highlight the contents.

2) The introduction turns out to be not very fluid: we appreciate the meticulous characterization of all the studied genes, but we invite you to improve the fluidity of this part.

3) As mentioned in the introduction, mitochondria are fundamental organelles involved in many biological processes like ATP production and apoptosis.

In glioma cells, the necroptosis process is also present and actively studied. We strongly suggest you read and cite it in your work (DOI: 10.1038/s41420-022-00974-x )

4) We encourage you to double check your manuscript font as some variances were found. We also ask you to specify why OGG12 is sometimes written as OGG1-2α.

5) The resolution of table 2 is too low. It results blurred and pixelated. Please, check and improve the quality of this image.

6) All your figures require major improvements and they are all presented in different sizes, making them visually chaotic.

6.1) in Figure 1, your graphs are not uniform. Some histograms have bold outlines while other have not. Also make sure your titles on the y axis have all the same character size.

6.2) In Figure 2, figure 3F, figure 5 and figure 6 are too small and difficult to read. We ask you to make them bigger and clearer for the reader.

6.3) Similarly, figures 7 and 8 have small tables under the graphs. Please, improve their resolution and size.

7) We recommend you to to standardize the letters indicating the various images of the panels. Some are bigger, while other are smaller and they need to be aligned in order to make your panel cleaner. Furthermore, we advise you to choose the same square shape for all the letters.

Reviewer #2: The authors have submitted a ms aimed at demonstrating deregulation of several genes resulting in

increased oxidative stress and DNA damage related to worse survival of patients with glioma

The authors also provide evidence that mitochondrial sirtuins and sirtuin-related genes can play

a role of diagnostic and prognostic biomarker for this neoplasm

The ms is clear and the discussion consistent with the results

However the authors should provide more details on some aspects of their work:

Can epileptic patients be considered "healthy controls" with no abnormality affecting the deregulation of genes and the pathways the authors show to be involved ? In which occasion and why the samples have been obtained from the epileptic patients ?

Why the authors di not carry on any seahorse analysis to achieve a better understanding of the metabolic abnormalities ?

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Reviewer #1: No

Reviewer #2: Yes: Luciano Mutti

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Attachments

Attachment

Submitted filename: PONE-D-22-26011.docx

Oncometabolic Role of Mitochondrial Sirtuins in Glioma Patients.

PONE-D-22-26011R1

Dear Dr. Ishrat Mahjabeen,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Erika Di Zazzo

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors have provided the manuscript with all the suggestions requested. All the comments were made in order to improve the fluidity of the text and to achieve images with higher resolutions.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

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