PONE-D-22-24567Evaluating the screening value of serum light chain ratio, β2 microglobulin, lactic dehydrogenase and immunoglobulin in patients with multiple myeloma using ROC curvesPLOS ONE

Dear Dr. Xu Linglong,

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Reviewer #1: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: No

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Reviewer #1: This is a retrospective study of 303 suspected MM patients. The finding shows the triple combination (sLC

ratio+β2-MG+Ig) had a sensitivity of 94.20% and a specificity of 86.75%. I have some comments

1. Due to small number of cases, please provide smaple size caluculation to eveluate that the number of patients is optimal for this analysis

2. Patient in MM group shold be exclude patient with SFLC ratio >100 to compare with non-MM group because this study is objective to find factor predictive of MM dignosis. But patient with SFLC >100 can diagnosis of MM and do not need other lab parameters (β2-MG+Ig) to predict the diagnosis.

3. Beta-2 microgloulin might be false high in patients with renal impairment. Therefore please provide baseline creatinine and renal function between both groups.

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Reviewer #1: No

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Evaluating the screening value of serum light chain ratio, β2 microglobulin, lactic dehydrogenase and immunoglobulin in patients with multiple myeloma using ROC curves

PONE-D-22-24567R1

Dear Dr. Xu Linglong,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Vipa Thanachartwet, M.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

All issues raised by the reviewer have been addressed.

Reviewers' comments: