PONE-D-22-25921Detection of Enterobius vermicularis in archived formalin-fixed Paraffin-Embedded (FFPE) appendectomy blocks: It's potential to compare genetic variations based on mitochondrial DNA (cox1) genePLOS ONE

Dear Dr.  Kazemirad,

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PLOS ONE

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Additional Editor Comments :

Dear Authors

We have received the paper entitled " Detection of Enterobius vermicularis in archived formalin-fixed Paraffin-Embedded (FFPE) appendectomy blocks: It's potential to compare genetic variations based on mitochondrial DNA (cox1) gene". Based on the evaluation, the submitted paper has such exciting data, especially on FFPE specimens which needs to be more studied nowadays. However, the major points raised by the Referee and the AE, are given below. Please address all raised issues point by point in the revised submission.

1-Why have you selected only coxi? Why not other genes included? Have you ever tested to be amplified by the PCR?

2-Figure 1, the scale should be in the same size and font.

2-Figure 2, is of low quality, and the authors mentioned 12 positive bands by PCR; why are only a few of them included?

3-Figure 4 is in low quality

4-In figure 5, the authors need to delete all ".1" from the accession numbers. Also, the worked sequences with accession numbers of the Iranian specimens should be given by the species name and in bold. You should also indicate the clade and discuss the clade with which your sequences are grouped with.

5- In discussion, you need to discuss your sequences based on the histology, morphology, and similarity or dissimilarity with the relevant sequences.

6-The references need to be adjusted in font and style with PLoS One.

7- the rest of the comments are included in the attached file.

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript including data of mtDNA of preserved specimens from Iran. The manuscript includes such interesting data, but it needs to be improved.

1-why authors selected coxi? Why other genes not been selected?

2-detail method of extraction from formalin fixed specimens not explained well.

3-in line 304, the author mentioned data not shown. Why? it should be presented or provided as supplementary file.

4-line 271 to 272 need to be revised, it is unclear.

5-English style needs to be improved.

6-The figure are not in high quality and should be improved.

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Reviewer #1: No

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Attachments

Attachment

Submitted filename: Enterobius 18th September final (1).docx

Detection of Enterobius vermicularis in archived formalin-fixed paraffin-embedded (FFPE) appendectomy blocks: It's potential to compare genetic variations based on mitochondrial DNA (cox1) gene

PONE-D-22-25921R1

Dear Dr. Kazemirad,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Ebrahim Shokoohi

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

The paper has been improved. The concerns raised by the AE and Referee were addressed.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: All comments and questions raised were addressed and the authors improved the paper. This paper has interesting information on the parasite and as the authors worked on the mtDNA as one of the important marker in this filed, the paper provide good information for the researchers and all readers interested.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

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