PONE-D-22-22747Changes in the Vitreous Body after Experimental Vitreous Hemorrhage in Rabbit: An Interdisciplinary StudyPLOS ONE

Dear Dr. Yan,

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors nvestigated the changes in vitreous body after vitreous hemorrhage and assess its prognosis 18 from the perspective of vitreoretinal interface

Currently, medicine has gone through moments of great renewal(1), and advanced methods s have been used(2). Many complications have been observed in the medical practice, such as the vitreous, may also lead to retinal detachment(3).

An optic nerve is an outward form of the diencephalon during embryogenesis, wrapped by a nerve sheath that is derived from three layers of meninges and protrudes toward the orbit(4). As a consequence of this communication, cerebrospinal fluid (CSF) can transfer freely between the intracranial and intraorbital subarachnoid space(5). ICP is transmitted within the optic nerve sheath to obstruct the venous drainage from the eye. Intra-ocular pressure (IOP) was likely elevated after injection blood or serum. Experimentally, a low volumes of intravitreal blood may also cause toxicity in rabbit retina. IOP is regulated primarily through changes in aqueous humor formation and outflow. .

Similarity analyze reveals that the manuscript is similar to other article(without references) ata rate 35%. It should be less than 30%.

There are to aricle which are similar at a rate 8%. One of them is unpublished PDF.

Other is an article (doi: https://doi.org/10.1101/2020.07.09.20150136) but this article was not aslo cited.

The aim of authors is notclear in the introduction section, but they wrote in first sentence of abstract. It should be emphasize in the introduction section.

In order to understand the changes after vitrous hemorrhage, the authors performed experiments in a rabbit model by injection of blood or saline, as well as control animals that did not undergo, so a SHAM group was used, but the finding of this group was not given in the result section

References

1. Gasenzer ERER, Kanat A, Ozdemir V, Neugebauer E. Analyzing of dark past and bright present of neurosurgical history with a picture of musicians. British Journal of Neurosurgery. 2018 May;1–2.

2. Kanat AA, Tsianaka EE, Gasenzer ERE, Drosos EE. Some Interesting Points of Competition of X-Ray using during the Greco-Ottoman War in 1897 and Development of Neurosurgical Radiology: A Reminiscence. Turk Neurosurg. 2021;accepted.

3. Findik H, Kanat A, Aydin MD, Cakir M, Ozmen SA, Okutucu M, et al. Describing a New Mechanism of Retinal Detachment Secondary to Ophthalmic Artery Vasospasm following Subarachnoid Hemorrhage: An Experimental Study. J Neurol Surgery, Part A Cent Eur Neurosurg. 2019 Aug;80(6):430–40.

4. Kazdal H, Kanat A, Findik H, Sen A, Ozdemir B, Batcik OE, et al. Transorbital Ultrasonographic Measurement of Optic Nerve Sheath Diameter for Intracranial Midline Shift in Patients with Head Trauma. World Neurosurg. 2016 Jan;85(1):292–7.

5. Kanat A, Kazdal H, Findik H. In Reply to Letter to the Editor Regarding “Transorbital Ultrasonographic Measurement of Optic Nerve Sheath Diameter for Intracranial Midline Shift in Patients with Head Trauma”. World Neurosurg. 2019 Oct;130:586.

Reviewer #2: This study investigated the changes in vitreous body after vitreous hemorrhage and evaluated its prognosis from the perspective of vitreous interface. The vitreous hemorrhage model in this study is novel, and interdisciplinary research is very meaningful. This experiment designed to explore the changes in the vitreous body after vitreous hemorrhage (VH) in rabbits. They tested IOP, absorption, vitreous retina interface (VRI) and vitreous properties. The results could verify the purposes. Further research should focus more on the biomechanical parameters and electrolytes that significantly changed in the vitreous hemorrhage model.

There are number of minor errors and require the revision:

1. The motivation of study require to add to Introduction and the implication for clinical application also need to provide in Discussion.

2. Line 36 of the article: “and then subsequently…”, the then should be dropped.

Line 85, “fixed” should be replaced with “cured”

3. The English writing needs to improve by professional editor.

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Reviewer #1: Yes: Ayhan Kanat

Reviewer #2: No

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PONE-D-22-22747R1Changes in the vitreous body after experimental vitreous hemorrhage in rabbit: an interdisciplinary studyPLOS ONE

Dear Dr. Yan,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Feb 16 2023 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Andrzej Grzybowski

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: No

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: No

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: This interdisciplinary study focused on the effects of hemorrhage on biomechanics, biochemistry and ultrastructure of vitreous body. Vitreous hemorrhage is an important issue, because it may lead to persistent loss of vision(1). In this study, the eyes of rabbits were assigned into two groups: study group (right eyes) – full autologous blood injected, and control one (left eyes, evaluate possible adverse effects of ocular trauma or exogenous fluid) – injected with equal volumes of saline in the same manner. No blank control animals that did not undergo.

The author agrre that a control group is not required to justify their statements.

Is there any previous study that use this method?

If here is, I recommend them to cite that study.

If not, this situation should be noted in the limitation section.

The ultrastructure images of the control group (saline injected) were supplemented. It is difficult to accept the saline injected animal as a control group animal.

As part of the central nervous system, the optic nerve is surrounded by a distensible subarachnoidal space, designated as the optic nerve sheath(2). As a consequence of this communication, CSF can transfer freely between the intracranial and intraorbital subarachnoid space(3). This communication may be the reason why papilledema be a common neuro-opthalmological exam finding of intraocular pressure elevation. In this study, after intravitreal injection of blood or saline , IOP did not fluctuate significantly. How can the authors explain this situation?

Findik et al found that retinal detachment is directly related to the degree of vasospasm in the ophthalmic artery(1). I have a concern about the author’ suggestion about that the retina can dynamically maintain the blood flow rate constant. What do you think the authors about the vasospasm in the ophthalmic artery? The manuscript require minor revision

References

1. Findik H, Kanat A, Aydin MD, Cakir M, Ozmen SA, Okutucu M, et al. Describing a New Mechanism of Retinal Detachment Secondary to Ophthalmic Artery Vasospasm following Subarachnoid Hemorrhage: An Experimental Study. J Neurol Surgery, Part A Cent Eur Neurosurg. 2019 Aug;80(6):430–40.

2. Guvercin AR, Besir A, Kanat A, Yazar U, Findik H. Interesting Negative Correlation between Transorbital Optic Nerve Sheath Diameter and Evans’ index Values; Can it Be Predictive for Failure of Endoscopic Third Ventriculostomy? Int J Neurosci. 2022 Sep;1–11.

3. Kazdal H, Kanat A, Findik H, Sen A, Ozdemir B, Batcik OEOE, et al. Transorbital Ultrasonographic Measurement of Optic Nerve Sheath Diameter for Intracranial Midline Shift in Patients with Head Trauma. World Neurosurg. 2016 Jan;85(1):292–7.

Reviewer #2: All comments have been addressed. This manuscript is technically sound, and the data support the conclusions.

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If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Ayhan Kanat

Reviewer #2: No

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Changes in the vitreous body after experimental vitreous hemorrhage in rabbit: an interdisciplinary study

PONE-D-22-22747R2

Dear Dr. Yan,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Andrzej Grzybowski

Academic Editor

PLOS ONE

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