PONE-D-22-18593Phenotypic and Genotypic screening of fifty-two rice (Oryza sativa L.) germplasms for desirable cultivars against blast diseasePLOS ONE

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Additional Editor Comments:

In view of the comments of the reviewers and based on my own assessment, I recommend the manuscript for a major revision

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The article is well written but I have few observations: (i) the natural screening is ok for testing the field resistance but to know the proper resistant line it is essential to go for artificial screening. Why the authors did not go for that? (ii) Neck blast is highly dependent on favorable weather condition coinciding with the flowering time. You have taken different breeding lines which must have different durations so, there is a chance that the lines which are showing resistance may be disease escape. (iii) Can we report breeding lines which we don't have IET numbers?

Reviewer #2: The paper is written in a detail way and is useful to identify the potential donors for leaf and neck blast. MAS utilization with the genes for neck and leaf blast will be helpful to develop blast resistant genotypes. The authors explained the research paper in a detail way and understandable for the researchers involved in rice breeding.

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Reviewer #1: No

Reviewer #2: Yes: Dr. S V Sai Prasad

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Attachments

Attachment

Submitted filename: PONE-D-22-18593 (1).pdf

Phenotypic and Genotypic screening of fifty-two rice (Oryza sativa L.) germplasms for desirable cultivars against blast disease

PONE-D-22-18593R1

Dear Dr. Hossain,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Muhammad Abdul Rehman Rashid, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: The author has incorporated all the minor suggestions made and able to answer the issues raised by both the reviewers. The information generated properly and was written in a detail way and is useful to identify the potential donors for leaf and neck blast. MAS utilization with the genes for neck and leaf blast will be helpful to develop blast resistant genotypes. The authors explained the research paper in a detail way and understandable for the researchers involved in rice breeding.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #2: No

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