PONE-D-22-21240Lack of the Ig cell adhesion molecule BT-IgSF (IgSF11) induced behavioral changes in the open maze, water maze and resident intruder test

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Reviewer #1: Partly

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: The manuscript by Montag et al., have demonstrated the impact of BT-IgSF in various behaviour paradigms. It is a novel finding and has implications in understanding diseases induced by this particular mutation. They authors conclude by stating that specific behaviours are modified by the lack of BT-IgSF and demonstrate a contribution of BT-IgSF to network functions, which they have not shown it experimentally. The manuscript will be strengthened if they could show the below mentioned points.

1.The authors have mentioned about the role of AMPAR in the behavioural abnormalities they have observed in this mouse model. However, they should have showed using electrophysiology or Immunohistochemistry or immunoblot techniques. Furthermore, given the role of AMPARs in memory formation and lack of it, they should have done one or two experiments on the impact of these mutations on basal synaptic transmissions.

2.The authors extrapolate the results to altered function of gap junction mediated communication between astrocytes and the hippocampus. However, the authors should have demonstrated these hypotheses using IHC.

3.Does this mutation impact sociability in these mice?

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Reviewer #1: Yes: James Chelliah

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Lack of the Ig cell adhesion molecule BT-IgSF (IgSF11) induced behavioral changes in the open maze, water maze and resident intruder test

PONE-D-22-21240R1

Dear Dr. Montag,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Jamuna Subramaniam, Ph.D

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

I am glad to state that your manuscript is accepted based on the correlation between Bt-IGsF1 deficiency altering behaviors in these mice.

Reviewers' comments: