PONE-D-22-28565Blood concentrations of mediators released from activated neutrophils are related to the severity of alcohol-induced liver damage. --PLOS ONE

Dear Dr. Kasztelan-Szczerbińska,

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Additional Editor Comments:

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Abstract

It is not usual to use the name "conventional" markers of inflammation. Please replace with a more adequate term. NE was related to MELD and mDF scores. What do you mean by this?

Keywords?

Blood biomarkers. Broad term, should be replaced

Introduction

Very clear and concise. However, this is the first time I've encountered a goal like this. He is really clear, however, the questions asked are not usual for the original article. If this is the policy of the journal, then it is ok. If not, it is necessary to modify the questions in, for example, aim: 2. Ln 86-87. Conducted study... This should be transferred to Material and methods

Material and methods

Ln 139 Please check the names of the parameters and add the correct abbreviations or description of the abbreviations where they are missing. For example: alanine aminotransferase is not A1AT, but ALT. Guanosine-5'-triphosphate is GTP. In your case it is gamma-glutamyl transpeptidase. Do you mean transaminotrasferase (GGT)? Why did you mention alkaline phosphatase in the assessment of liver function, when it is an organospecific enzyme for bones?

What is INR?

Ln, 141 it is necessary to equalize the WBC count and RBC count.

Abbreviations are missing again.

Ln 145 Replace the word conventional.

I believe that the methods should be classified into several groups. Please form adequate titles and subtitles. You can't write together about blood and serum parameters, then there is a division of patients, certain criteria and symptoms….

Table 2. GTP, please correct this cardinal error. Here it is now ALT, and in methods A1AT. What does the mark kom/uL represent? If the international normalized ratio (INR) is the unit for prothrombin time, there cannot be an INR in the table. It's the same for you if you wrote mmol/L instead of the glucose value. Ln instead of 700 x g specify rpm.

Table 4. Please enter a numerical value for each p value, and not combine p and significant levels. Place a mark next to the p value and mark the level of significance (p<0.000) under the table.

Tables 5 and 6 are not described. Remove n from Table 6 to make the table smaller.

Please eliminate the green coloring and mark significant differences with *.

The description of the images is also not given. It looks like you presented the data as figures next to the table.

The figures are not described at all. It is necessary to standardize all tables. It is better to eliminate the green ones, put a sign * and write an explanation for significance under the table. In general, a lot of data in the results is displayed chaotically.

3.5. There are no data.

Discussion

The first page of the discussion (ln 359-385) is a general story. This could have been the Introduction. The discussion is well written.

Reviewer #2: Thanks so much for trust me to review this article

I have just one recommendation to the authors is :

the type of the study must mention in the abstract

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Reviewer #1: No

Reviewer #2: Yes: My name : Dr.Zubaida Falih Alzubaidi

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Blood concentrations of mediators released from activated neutrophils are related to the severity of alcohol-induced liver damage. -

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PONE-D-22-28565R1

Dear Dr. Kasztelan-Szczerbińska,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Gulali Aktas

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewer comments were addressed adequately in the revised paper. I recommend publication of the manuscript in its current form.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: After carefully reading the author's comments, I believe that significant changes have been made in manuscript. I suggest that the manuscript be published.

Reviewer #2: I have just one recommendation to the authors is :

the type of the study must mention in the abstract

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

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