PONE-D-22-26732The study of GSDMB in pathogenesis of psoriasis vulgarisPLOS ONE

Dear Dr. Li,

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Partly

Reviewer #3: No

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: No

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Xiaojuan Ji et al. performed an interesting study on the role of GSDMB in the pathogenesis of psoriasis. However, there are several issues that should be addressed.

Abstract

Line 25 “GSDMB is a member of the GSDM (gasdermin) family” should be reformulated as follows “Gasdermin (GSDM) B is a member of the GSDM family”

Introduction

Line 50 The abbreviation PS for psoriasis is not useful. The abbreviation PV for psoriasis vulgaris is sufficient.

Line 68 “However, few report is about the relationship between keratinocytes and GSDMB”. The authors should provide bibliographic references and improve grammar, I suggest 'few reports are on'.

Line 70 “abnormal differentiation of hyperkeratosis” Psoriasis is characterized by abnormal differentiation of keratinocytes. Line 71 “incomplete keratosis”. Psoriasis is characterized by parakeratosis that refers to incomplete maturation of epidermal keratinocytes. I consider that this phrase is misleading and the authors should rephrase it.

Materials and methods

Line 80 “Skin lesions or skin of PV patients …” This statement is misleading.

Results

All tables should be mentioned in the text. The resolution of Figure 1 should be improved.

Discussion

When authors use expressions such as 'studies have shown', they should indicate several references, not just one (e.g. line 237, line 257, etc). Please review the entire manuscript.

Line 238 “GSDMB located at 17q12-21”. The authors mention it again at line 249.

Line 240 “There is growing evidence that there is a correlation between immune-mediated inflammatory diseases and psoriasis”. The authors should provide bibliographic references.

Line 274, 278 – The reference number should be moved to the end of the sentence.

The English language should be revised.(e.g. line 253-255).

Reviewer #2: The authors investigated the role of GSDMB in the pathogenesis of psoriasis. This brings new insights into the pathophysiology of the psoriasis and clinical investigation of the GSDMB. Here are some comments to improve the impact of the article.

Minor comment:

1) The author mentioned GSDMB’s role in cell pyrolysis process (Line 26), it will better to clary the potential effects of pyrolysis on psoriasis, since there are no reports on pyrolysis in psoriasis. Do you mean proptosis? If so, please correct it.

2) Line 102, CO2 should be CO2

3) Lines 265-266, the author said “GSDMB is not involved in the pathogenesis of psoriasis by affecting keratinocyte proliferation/apoptosis”, but the results showed GSDMB can promote HaCaT proliferation and inhibit apoptosis. Can author verify the results and conclusion?

4) References 12, 22, and 24, the author names are capital, inconsistent with other ones.

5) The figures are low-quality, please replace higher ones.

Reviewer #3: In this manuscript, the authors demonstrate that The level of GSDMB protein in the skin lesions of patients with psoriasis vulgaris was lower than that in normal skin tissues and demonstrate that the down-regulation of GSDMB expression can inhibit cell proliferation and promote cell apoptosis.

However, psoriasis is a kind of inflammatory disease, if you don’t test function of this gene in inflammation, we can’t tell its importance in this disease. What’s more, i do think you should build the psoriasis model in your experiments to test the gene function.

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Reviewer #1: No

Reviewer #2: No

Reviewer #3: No

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Submitted filename: Comments-Plos one.docx

The study of GSDMB in pathogenesis of psoriasis vulgaris

PONE-D-22-26732R1

Dear Dr. Li,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Yi Cao

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Manuscript has been adequately improved. The authors have addressed all suggestions; I consider the manuscript could be published, according to the Editor's choice.

Reviewer #2: The authors addressed all the comments. There are no additional comments for the author or any concern about dual publication, research ethics, or publication ethics.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

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