PONE-D-22-15957Genetic diversity of whitefly species of the Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) species complex, associated with vegetable crops in Côte d'Ivoire.PLOS ONE

Dear Dr. N'cho,

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Yunzhou Li

Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

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Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Partly

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: No

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Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

Reviewer #4: Yes

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Reviewer #1: Yes

Reviewer #2: No

Reviewer #3: Yes

Reviewer #4: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Genetic diversity of whitefly species of the Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) species complex, associated with vegetable crops in Côte d'Ivoire.

Authors described the genetic diversity of the whitefly species of the Bemisia tabaci species complex, associated with vegetable crops in Côte d'Ivoire. In my opinion manuscript in present form is not accept to publish in Plos One. First of all in presented paper there is no answer of question why this research are important and why should be presented to international audience. Moreover, reading present paper, audience might have impression that problem is more local, not international. Authors must put more effort to the Introduction and Discusion section. I recomand the major revision of manuscript.

Introduction section - this section MUST BE improved. After reading the audience will not know why this research are important. Among others information, there is lack of goal of present work and hypothesis. Moreover I think that there is bad style of citation used in all manuscript.

L68 - Why did you use this localisation?

L104 - Why only female? Was there any differences in ratio male:female in particular localisation?

L115 - Please add some information about what kind of polimerase you used and in in which concentration?

L119 - Please add the information about electrophoresis of the PCR products

I recommend make some statistic analysis, like PCA analysis to compare the results

Discussion section: should be prepare better, there is lots of information which are not the result of conduction experiment, and at the some time lack of proper results discussion

Reviewer #2: I would like to make the following suggestions to the authors in order to improve the manuscript so that it is ready for publication:

1) Align the manuscript properly (line numbers, paragraphs, italic characters etc.). Read author guidelines carefully.

2) There are a lot of prepositions and Grammarly mistakes. Please use some relevant software to check these things or seek some help from a good English spoken person or teacher.

3) Introduction is very short. Please provide enough relevant information regarding insecticides resistant, diversity, and morphology.

4) In keywords under abstract: do not repeat the common name or scientific name of the same species (use one). Also, consider replacing with more informative and specific words e.g. genetic diversity, species diversity, Phytovirus etc. instead of vegetables, whitefly, and ecology.

5) Line:89 "If treatments were applied then how did you differentiate between the treated or untreated species? Did you take that treated species also into the account. if yes then explain".

6) Line:71-73 "it is irrelevant to mention the environmental conditions. please cite some suitable references for this or highlight these things within a figure or graph with suitable legends.

7) Line:82 "correct the spellings of whiteflies and wherever required in the whole manuscript".

8) Line 106 "Why females from site 6 or 7 were not selected"?.

9) There is misprinting in the headings of the table. make sure to correct them.

10) Line 242: Make the scientific name of the species italic and wherever required in the whole manuscript".

11) Line 255: suggest changing to “Regarding" instead of with regard to.

12) Line 335: suggest changing to "However" instead of nevertheless

13) References are very poorly written. Cite references properly. Scientific names should be italic.

Reviewer #3: In this MS., authors investigated the species diversity and their genetic diversity and structuring on smaples from vegetable crops in the major tomato production areas of Cote d’Ivoire. They found four whitefly species, including MED ASL, MED Q1, SSA1 and SSA3 by using mtCOI marker. And, they further revealed MED ASL is the main species which is the main phytovirus vector in the Ivorian vegetable cropping areas. These results provide useful information for monitoring whitefly population in Cote d’Ivoire. The methods and analysis sounds good. The conclusion and discussion are also solid and nice. The writing is very nice and clear. I think the MS is ready to published.

Reviewer #4: This study investigated the distribution and biotypes of whitefly, Bemisia tabaci in Côte d'Ivoire. The MED ASL was identified as the most popular biotype on variant vegetable plants. The analyses of the data are inadequate and the figures are not clearly displayed. The language need to be modified carefully.

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Reviewer #1: No

Reviewer #2: No

Reviewer #3: No

Reviewer #4: No

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PONE-D-22-15957R1Genetic diversity of whitefly species of the Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) species complex, associated with vegetable crops in Côte d'IvoirePLOS ONE

Dear Dr. N'cho,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Oct 07 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Yunzhou Li

Academic Editor

PLOS ONE

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Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Additional Editor Comments:

please answer all question in manuscript and put reference in right place,

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Reviewers' comments:

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Genetic diversity of whitefly species of the Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) species complex, associated with vegetable crops in Côte d'Ivoire

PONE-D-22-15957R2

Dear Dr. N'cho,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Yunzhou Li

Academic Editor

PLOS ONE

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