PONE-D-22-15319Natural selection drives the evolution of mitogenomes in AcrossocheilusPLOS ONE

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Reviewer #1: Partly

Reviewer #2: Partly

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Reviewer #1: N/A

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: No

Reviewer #2: No

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5. Review Comments to the Author

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Reviewer #1: 1. The current work cannot fully support "ATP6 was significantly correlated with a mean diurnal temperature range" and "Ka/Ks ratio of COX2 was strongly associated with several precipitation-related variables". The authors are required to provide further analysis or sufficient evidence to support these views.

2. The authors declared that their work provides a foundation for integrating the mitochondrial genome into future investigations of how these species may respond to ongoing global climatic changes. I don’t understand how did they conclude that?

3. The author selected 25 species with the closest relationship to construct a phylogenetic tree, just for selection analysis? When the author performed phylogenetic analysis and Ka/Ks analysis, it is necessary to expand the research scope of species, and compare the differences between different taxa in combination with the traits of species. Obviously, this research is not enough.

4. Lines (78-82), please provide a bibliography or citation source.

5. Many researches have focused on the environmental adaptation of organisms. A previously published mitochondrial study (DOI: https://doi.org/10.1186/s12862-021-01803-y) can provide a reference for this study.

6. It is recommended that the results of the selection analysis should be presented graphically.

7. Line 307, "<<" should be "<".

8. Lines (309-311) “The differing mutation rates (μRelative) that we detected across different genes may result from differences in the strength of purifying selection due to functional constraints”. Is there sufficient evidence to support this conclusion? If yes, please list it. If not, please delete it.

Reviewer #2: This is fine study, and some data is valuable. However, I am not quite sure that the current study fits to the journal scope and standard because this is very general study which is not something new.

1. “This genus has 26 species, which have a center of diversity (21 species) in southern China and commonly occur in middle and/or lower reaches of river drainage”. But in this paper, the authors only chose 25 published sequences of 14 species to analyze. It is better to add more new sequenced species to confirming the findings.

2. In this paper, although the phylogenetic tree with high bootstrap, if we add other species, the topology will change, and the authors did not describe the taxonomy of the genus in the introduction.

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Reviewer #1: No

Reviewer #2: No

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Natural selection drives the evolution of mitogenomes in Acrossocheilus

PONE-D-22-15319R1

Dear Dr. Gao,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Bi-Song Yue, Ph.D

Academic Editor

PLOS ONE