PONE-D-21-349902-Bromopalmitate decreases spinal inflammation and attenuates oxaliplatin-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunctionPLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. the authors made all data underlying the findings described in their manuscript fully available without restriction. The manuscript is presented in an intelligible fashion and written in standard English.

Reviewer #2: Manuscript: 2-Bromopalmitate decreases spinal inflammation and attenuates oxaliplatin-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunction

Authors: Zhi-Bin Dong, Yu-Jia Wang , Meng-Lin Cheng , Bo-Jun Wang, Hong Lu , Hai-Li Zhu , Ling Liu, Min Xie.

ID: PONE-D-21-34990

Overall recommendation:

Accept after addressing minor revisions

General comments:

The paper by Zhi-Bin Dong and colleagues add another important element regarding the toxicity of platinum derivate Oxaliplatin and on the use of 2-Bromopalmitate to counteract these effects.This manuscript represents a good starting point as reference data for future studies on this topic. Despite the present study has a good experimental idea, it needs revisions both for contents and structural organization in order to improve the quality of the manuscript. Some acronyms are missing, written in full, eg: DCFH-DA and PWT, please add them in the text.

Keywords:

It would be advisable to change the keywords; some of these are also present in the paper's title.

Background:

It would be appropriate to include more information about oxaliplatin toxicity, so I suggest that the authors include the following reference on zebrafish model in which many aspects related to oxaliplatin toxicity from both morphological and molecular points of view are emphasized. DOI: 10.3390/toxics10020081.

Materials and Methods;

Materials and Methods section needs revision:

It would be opportune in indicate the full addresses of the manufacturers from whom the materials used for conducting the experiments are procured, please standardize the whole section accordingly.

It would be desirable to improve the quality of the description of the methods used as well,I suggest taking a cue for the exposition of the Western Blot procedure from the following manuscript and insert the appropriate citation : DOI: 10.3390/life12010128

The authors need to implement the quality of even the purely technical sections ,for SOD measurement take a cue and cite: DOI: 10.3390/toxins13100710

Same for ROS assessment section, take a cue from the text and cite it: DOI: 10.3390/toxics9120344

The authors stated: “After TNF-α inducement and 2-BP treatment, C6 cells were loaded with MitoTracker Red CMXRos or DCFH-DA and incubated at 37 ℃ for 30 min”.It’s not clear what the author used for this assay, was MitoTracker Red CMXRos or DCFH-DA?,please revise.

Figures: I suggest the authors to arrange the legend figures ,sometimes there are letters corresponding to a photo in brackets,sometimes not,it becomes very difficult to follow what the authors are trying to describe

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Reviewer #1: No

Reviewer #2: No

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2-Bromopalmitate decreases spinal inflammation and attenuates oxaliplatin-induced neuropathic pain via reducing Drp1-mediated mitochondrial dysfunction

PONE-D-21-34990R1

Dear Dr. xie,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Rodrigo Franco

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Thanks for addressing all comments and corrections of the manuscript which represents a very good and informative research in this field

Reviewer #2: The authors answered all my comments and improved the quality of the manuscript which is now suitable for publication.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

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