PONE-D-22-05127Genetic diversity of Ralstonia solanacearum causing vascular bacterial wilt under different agro-climatic regions of West Bengal, IndiaPLOS ONE

Dear Dr. Roy Barman,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Dear authors,

In line with the reviewer comment I too find the reported study by Ghorai et. al 2022 very interesting.  The research work reported here contains extensive field work, laboratory work and bioinformatics analysis to reach the conclusions. I must appreciate the authors for this wonderful research.

However, I have some minor concerns, which are listed below.

Line no 22- diseases can be corrected to disease

Line no 23- (Smith 1896) Yabuuchi et al. 1995 citation may not be required in the abstract

Line no 89- “reacting positive” change wording

Line no – “32 ±1°C or 27 ±1°C day/night” are the authors want to mean day temperature 32 ±1°C and 27 ±1°C as night temperature? Clarify and if necessary modify.

Line no – 164“Purification of extracted DNA was made by centrifugation at 15493g for 5 min” I think better not to refer the process as purification since it is just a simple centrifugation step.

Line no – 164  “16S-23SITS”, after 23s give a space

Line no – 737- “This Red and Lateritic Zone of West  Bengal shares the major border with solanaceous vegetable growing neighbouring state  Jharkhand, India.” Rewrite the sentence.

Line no 317: Pathogenecity test- Among 36 isolates, results of all the isolates are not mentioned properly. Please rectify.

Line no 462: “No values were obtained for non-synonymous selection” is it appropriate?

Please check.

Best Regards

Niraj Agarwala

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Kind regards,

Niraj Agarwala, Ph.D.

Academic Editor

PLOS ONE

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Funding Authority: Science and Engineering Research Board (SERB), Department of Science and Technology, Government of India

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Bacterial wilt in plants caused by Ralstonia solanacearum is prevalent in India. Understanding the pathogenicity potential of natural isolates in different hosts and grouping them by molecular analysis is an important study, which has been carried out by the authors. I found the methodology followed is rigorous and very interesting findings in the manuscript. I recommend its pub;ication.

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Reviewer #1: No

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Genetic diversity of Ralstonia solanacearum causing vascular bacterial wilt under different agro-climatic regions of West Bengal, India

PONE-D-22-05127R1

Dear Dr. Roy Barman,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Niraj Agarwala, Ph.D.

Academic Editor

PLOS ONE

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