PONE-D-22-18944Insight into the Effect of Low Temperature Treatment on Trichome Density and Related Differentially Expressed Genes in Chinese CabbagePLOS ONE

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: No

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript entitled ‘Insight into the Effect of Low Temperature Treatment on Trichome Density and Related Differentially Expressed Genes in Chinese Cabbage’ provide valuable information related to trichome formation in Chinese cabbage.

I have few suggestions for the authors before this research get publication.

1: The English writing is very difficult to understand such as ‘Now, all Raw dates obtained by sequencing have been submitted to NCBI, login number: PRJNA842790.’ which might be ‘Raw data’. Therefore, the manuscript needs native English editing.

2: The references are not match with the citation in MS correctly, such as ‘Chinese cabbage trichomes play an obvious role in increasing leaf thickness to reduce heat of epidermis [26], loss of water [27], and defense against insect pathogen invasion and mechanical injury [28-29], but it also affects the consumption quality of cabbage.’ The references of 26 and 27 the authors listed are not mentioned about Chinese cabbage trichomes at all. Check it please.

Ref.

26. Kenzo T, Yoneda R, Azani MA, Majid NM. Changes in leaf water use after removal of leaf lower surface hairs on Mallotus macrostachyus (Euphorbiaceae) in a tropical secondary forest in Malaysia. Journal of forest research. 2018;13(2): 137-142. doi: https://doi.org/10.1007/s10310-008-0062-z.

27. Hamaoka N, Yasui H, Yamagata Y, Inoue Y, Furuya N, Araki T, et al. A hairy-leaf gene,BLANKET LEAF, of wild Oryza nivara increases photosynthetic water use efficiency in rice.Rice. 2017;10(1): 20. doi: 10.1186/s12284-017-0158-1. Epub 2017 May 12. PMID: 28500411;PMCID: PMC5429320.

3: The authors need describe the materials and methods clearly, such as ‘CT and C are treated at room temperature, while LCT and LC are treated at low temperature.’ What are LCT and LC exactly? Moreover, give the numbers of seedlings in each treatment instead of ‘some of’ and ‘the remaining’.

4: ‘After 10 days of treatment at low and normal temperature, 3 cabbage plants were taken from each treatment and 3 leaves were selected from top to bottom.’ Pay attention to Chinese cabbage and cabbage which are totally different.

5: Table 1 need remove to results part.

6:The authors need rewrite the table and figure legends clearly.

7: the most important data analysis process of the final selected 9 genes for qRT-PCR validation is not mentioned in results part.

8: The abstract and conclusion need reorganize carefully.

Reviewer #2: This MS investigated the different expression genes related with trichome density of Chinese cabbage that treated with low temperature by transcriptome analysis. It is valuable for understand the regulating mechanism of trichome density in Chinese cabbage. Following is my suggestions for this MS:

The authors focused on the DEGs derived from comparison groups of ‘CT vs C, LCT vs LC and CT vs LCT’, while the underlying biological significances should be addressed and explained in this MS.

I did not find the result descriptions of Fig 10., looks like this part was missed in Results part or perhaps this is not a necessary Fig to this MS. Fig 11. should include the statistically significant analysis since these 9 genes are significant DEGs selected from RNA-seq data. The ‘Conclusion’ part actually is the ‘Discussion’, please modify accordingly and add the proper conclusion of this MS. In this MS, the low temperature treatment also affected the Chinese cabbage plant growth (size), how to distinguish the DEGs are truly related with trichome density regulation rather than involving in plant growth (size) or growth speed (slow or quick). Besides, this manuscript needs further editing for language and writing quality.

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Reviewer #1: No

Reviewer #2: No

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Insight into the effect of low temperature treatment on trichome density and related differentially expressed genes in Chinese cabbage

PONE-D-22-18944R1

Dear Dr. Cheng,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Maoteng Li

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: figure legends in Fig 1. A, Three visual fields (front, back, and edge) at room temperature (CT, C)of CT, C at room temperature and low temperature (LCT, LC); As I know, it should be adaxial and abaxial instead of front and back, check it please.

Reviewer #2: Minor issues: Line 39 should be '... on the surfaces of plants ...'; In Table 1, the gene ID should be written with italic format; Conclusion and discussion are very different sections in the main text of a manuscript, Line 260 should be Discussion rather than Conclusion.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

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