PONE-D-22-00070Morpho-molecular genetic diversity and population structure analysis in garden pea (Pisum sativum L.) genotypes using simple sequence repeat markersPLOS ONE

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: No

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

Reviewer #3: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Indeed the article is good but the authors must define

1- The innovation in the study with previous studies.

2- The future prospect and output of the study in which the study is based.

3- What is the take home message of the study. That means any genotype most promising or least promising for future studies and why?

Reviewer #2: The manuscript entitled “Morpho-molecular genetic diversity and population structure analysis in garden pea (Pisum sativum L.) genotypes using simple sequence repeat markers" by Sharma et al. is well written and organized.

Herein, the authors have demonstrated the genetic diversity among 56 garden pea genotypes using morphological descriptors, quantitative traits, and simple sequence repeat markers. I feel that the results provided by the authors will contribute to progress in the future garden pea breeding programs. As such, the manuscript contains a large amount of data. In general, the paper presents results that are novel and merits publication.

So, I strongly recommend the manuscript and can be accepted for publication after incorporating corrections as suggested below:

1. Figures 3 and 6 are not clearly visible.

Reviewer #3: Study by sharma et al on Morpho-molecular genetic diversity and population structure analysis in garden pea genotypes using SSR markers presents a very general overview of estimating genetic diversity. While the presentation and the text of the manuscript needs substantial improvement, my major concern is the rationale and relevance of the such study. In the era of omics, and with a better understanding of phenotype in during the past decade (a product of genotype x environment interaction), there could be opportunities to test this similar set of genotypes for specific phenotype and associated markers. Authors conclude their results with narrow genetic diversity among the genotypes and suggest gene pyramiding to enhance the genetic diversity? I could not understand it. Why not mutation breeding, which is a proven strategy to induce diversity in the such scenario. Overall, this study looks highly repetitive with the previous ones (that authors have mentioned frequently in the discussion section for e.g. page 17, line 313-315; line 318; page 18-19 line 358-362 and many more places) and doesn't add anything new to the existing information.

The sentences are often vague and at places incomplete (line 294). The presentation of results is complex while the outcome of the study is very generalised. Authors did not discuss, why the panel was chosen and what are the reasons or limitations with marker analysis to see the genetic diversity. Also, why morphological markers were chosen in study when it was clear at the first place that they are highly variable and do not help in genetic diversity analysis? these points needs very strong justification to make this article interesting for the readers. I can not recommend this manuscript for publication in its present form.

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Reviewer #1: No

Reviewer #2: No

Reviewer #3: No

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Attachments

Attachment

Submitted filename: Reviewer comment.docx

Morpho-molecular genetic diversity and population structure analysis in garden pea (Pisum sativum L.) genotypes using simple sequence repeat markers

PONE-D-22-00070R1

Dear Sir

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Mohar Singh, Ph.D. FNASc.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Congratulations, i have accepted your publication

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #3: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #3: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #3: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #3: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #3: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: I accept all the comments made by the reviewers. The reviewers have revised the manuscript as per the reviewers comments and now the manuscript is in perfect shape.

Reviewer #3: It could be better if the authors have shown the changes marked in the manuscript. Just wrting in response that we have addressed the comments doesn't look enough. No further comment on manuscript.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #3: No

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