PONE-D-22-20884Analysis of the mechanism of castor tolerance to Cd metal based on proteomics and metabolomicsPLOS ONE

Dear Dr. Fenglan,

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Kind regards,

Ying Ma, Ph.D.

Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The authors of manuscript “Analysis of the mechanism of castor tolerance to Cd metal based on proteomics and metabolomics” reported that the physiological results mainly emphasize the super-sensitive responses of castor plant roots to Cd stress and the effects of Cd stress on plants’ antioxidant system, ATP synthesis and ion homeostasis. Nevertheless these points need to be addressed by the authors:

1. It seems better to replace common name of castor by its botanical name in the title

2. Please add some important data of current study in the abstract section

3. Please write the keywords alphabetically. Don’t write those words as a keyword which is part of manuscript’s title. The first letter of keyword shall be either capital or small, keep uniformity

4. L91-92: Write “C” of copper and “M” of mercury in small letter

5. L98-100 and 101: Please rewrite the sentence, make statement clear

6. Study following articles to improve this section:

https://doi.org/10.1016/j.ecoenv.2021.112047

https://doi.org/10.1016/j.chemosphere.2021.132332

https://doi.org/10.1016/j.scienta.2020.109203

https://doi.org/10.15244/pjoes/80806

7. L105: Castor oil extracted from the seeds is not toxic and has medicinal use

8. L141: Please write the complete term before using its abbreviation. Afterwards write abbreviation of that term concerned. However, do not write an abbreviation at the start of a sentence. Use this practice throughout the manuscript

9. Please inform about the physichochemical properties of sowing media

10. Did you sterilize the seeds before sowing?

11. What was pot size material and size? How much soil added per pot?

12. L145: Write “p” of plants in small letter

13. L161-169: Please rewrite the sentence, make statement clear

14. L216: Please maintain uniformity while giving heading/ subheading

15. L270: Please incorporate required correction

16. L272: Write “C” of castor in small letter

17. Please improve the discussion section, use latest references

18. Please delete unnecessary detail from the conclusion, write this section in one paragraph

Reviewer #2: Experimental work is ok. But there is language issue throughout the manuscript. In the main pdf file, comments have been provided to improve the manuscript. Please find comments in the main pdf file in attachment.

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Reviewer #1: Yes: Nasim Ahmad Yasin

Reviewer #2: Yes: Imran Muhammad

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Attachments

Attachment

Submitted filename: PONE-D-22-20884_reviewer.pdf

Analysis of the mechanism of Ricinus communis L. tolerance to Cd metal based on proteomics and metabolomics

PONE-D-22-20884R1

Dear Dr. Fenglan,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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If your institution or institutions have a press office, please notify them about your upcoming paper to help maximize its impact. If they’ll be preparing press materials, please inform our press team as soon as possible -- no later than 48 hours after receiving the formal acceptance. Your manuscript will remain under strict press embargo until 2 pm Eastern Time on the date of publication. For more information, please contact onepress@plos.org.

Kind regards,

Ying Ma, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Authors of the manuscript "Analysis of the mechanism of Ricinus communis L. tolerance to Cd metal based on proteomics and metabolomics" have improved their manuscript.

Reviewer #2: The authors have addressed all the comments. I agree with acceptance of the revised version of the manuscript.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Nasim Ahmad Yasin

Reviewer #2: Yes: Muhammad Imran

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