PONE-D-22-08057Effect of Zanthoxylum bungeanum essential oil on rumen enzyme activity, microbiome, and metabolites in lambsPLOS ONE

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PLOS ONE

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: Figure 1D: ANOSIN (R na P) result are too small. Please provide a better figure.

Table 3. It would be best to standardize two digits after the dot

Line 277: How can you explain the predict genes of “Circulation system” , “Endrocne metabolic desease, Cytoesqueleton,....” if you provided the Microbial DNA sequencing?

LINE 285: The figure 4 legend needs to be improve.

Line 287: The same as before. Legend needs to better described.

Line 370: The same as before. Legend needs to better described

Reviewer #2: Line 20: The description "Subgroups:" sounds a bit strange, please check with the author.

Line 44: The numbers in the "CH4" area should be subscripts.

Line 72-74：What is the basis for the supplemental levels of EOZB of 0, 5, 10 and 15mg/kg in the experimental design? The trial lasted 52 days，why did choose this way?

Line 80-81: How was EOZB added? It is not stated in the dietary formula.

Line198-199：Which multiple-comparison is used?

Line213：Standard deviation rather than standard error should be used in Table 2.

Line 212,214：The P should be in italics.

Line 355, 342, 345, 351, 353, 354, 357, 359, 360: Spearman's analysis r value should be -0.5-0.5,please ask the authors to verify.

Line 377: References should be marked after "anti-inflammatory".

Line 388: References should be marked after the word "antioxidant".

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Reviewer #1: No

Reviewer #2: No

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Effect of Zanthoxylum bungeanum essential oil on rumen enzyme activity, microbiome, and metabolites in lambs

PONE-D-22-08057R1

Dear Dr. Wang,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Chun Wie Chong

Academic Editor

PLOS ONE

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