PONE-D-22-09656Cloning of Nine Glucocorticoid Receptor Isoforms from the Slender African lungfish (Protopterus dolloi)PLOS ONE

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Reviewer #1: Yes

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Reviewer #1: N/A

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: This study is from the well-respected group working on the evolution of MR and GR, two sister proteins of the steroid receptor. Previously, the authors have successfully cloned MR from P. dolloi, in which steroid hormone aldosterone first appeared. As a part of their series of studies, the authors now report the sequences of nine isoforms of P. Dolloi GR in detail, and discussed the alterations in these isoforms and species difference. The study adds new insights into the field and is of interest in terms of evolutionary perspective. My comments are as follows.

1. Future functional characterization of nine isoforms will add great value to the current study.

2. Information on relative expression levels of these isoforms would be helpful to the readers.

3. Can the authors make any inference about the reason why the lungfish has as many as nine isoforms? If so, please discuss.

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Reviewer #1: No

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Cloning of nine glucocorticoid receptor isoforms from the Slender African lungfish (Protopterus dolloi)

PONE-D-22-09656R1

Dear Dr. Baker,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Hubert Vaudry

Academic Editor

PLOS ONE

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