PONE-D-22-06967The G allele of the IGF1 rs2162679 SNP is a potential protective factor for any myopiaPLOS ONE

Dear Dr. Meng,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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James Fielding Hejtmancik, M.D., Ph.D.

Academic Editor

PLOS ONE

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Additional Editor Comments:

While both reviewers appreciate the contributions of this meta-analysis, but both have a number of comments that need to be addressed. Although some are fairly minor, there are some inconsistencies with regard to the description of the outcomes in the Results and Discussion section and the need to correct for multiple testing, or at least discuss this and why it is not necessary, as well as some problems with the figures and tables. Each of these need to be addressed.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: N/A

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: In this manuscript the authors reported The G allele of the IGF1 rs2162679 SNP is a potential protective factor for any myopia. the project is well conceived, the following comments for consideration:

1. in this study the comparisons were done between the different groups and SNPs using analysis of variance, the authors need to do multiple testing to reject false hypotheses.

2. In the Results rs2162679, "significant association" should be changed to "suggestive association."

3. In the Results , the paragraph descriptions of rs2162679 that supporting file should be (Table 1, Table 2) instead of (Fig 1, Table 2), this paragraph not related with Fig 1.

4. in Abstract and Discussion the author said rs2162679

was significantly associated with any myopia in the codominant model (GA vs. AA: OR

= 0.87, 95% CI: 0.76-1.00) and the dominant model (GG+GA vs. AA: OR = 0.88, 95%

CI = 0.78-1.00).

but in results the author said rs2162679 "there were no significant associations for the allelic model (G vs. A: OR = 0.93,

95% CI: 0.85-1.02, P=0.14), dominant model (GG+GA vs. AA: OR = 0.88, 95% CI =

0.78-1.00, P=0.05), recessive model (GG vs. GA+AA: OR = 0.99, 95% CI = 0.82-1.19,

P=0.92 and codominant model (GG vs. AA: OR = 0.92, 95% CI = 0.76-1.13, P=0.43).", this is inconsistency, authors need to correct that.

5. the authors need add in figure legend for Fig 1 and Fig 2 .

Reviewer #2: Meng B, et al., presented a manuscript performing a meta-analysis to investigate the association of IGF1 SNPs and myopia. Authors analyzed nine studies and included 7 SNPs in this meta-analysis. They found that Rs2162679 was significantly associated with myopia in both the codominant and the dominant model, and the G allele of the IGF1 rs2162679 SNP is a potential protective factor for any myopia. This manuscript is well written and minor revision is needed to address the following points:

1. Please include “meta-analysis” in the title

2. Add a reference to Page 7 Line 12-13 for the “Myopia has already become the second most common cause of legal blindness”

3. Page 8 Line 9: delete “[22]”

4. Table 1 is truncated on the right side and is not fully shown in the manuscript

5. Check the format of all references.

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Reviewer #1: No

Reviewer #2: No

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The G allele of the IGF1 rs2162679 SNP is a potential protective factor for any myopia: Updated systematic review and meta-analysis

PONE-D-22-06967R1

Dear Dr. Meng,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

James Fielding Hejtmancik, M.D., Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

The authors have addressed each of the reviewers' comments.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: the authors have been addressed all my concerns in the revised manuscript. overall, the results are complete and advance the field.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

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