PONE-D-22-10383Optimization and evaluation of a two-stage chromogenic assay procedure for measurement of emicizumab plasma levelsPLOS ONE

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Dear editor of PLOS ONE journal

The manuscript entitled “Optimization and evaluation of a two-stage chromogenic assay procedure for measurement of emicizumab plasma levels” was reviewed. The comments have been listed below:

1- The study has been designed well.

2- Different parts of manuscript have been written appropriately.

3) Which criteria were used for patient selection? Please explain completely.

4) How many healthy blood donors were selected for this study?

Regards

Reviewer #2: 1- The advantage of heat-inactivation should be explained more precisely

2. The authors should explain why the patients‘ samples are too

3. Please specify if the chromogenic FXa method was a kinetic or an endpoint method.

4. What are “general chemicals”. This should be specified when describing the details of

the assays. Accordingly, the first sentence can be eliminated.

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Reviewer #1: No

Reviewer #2: No

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Optimization and evaluation of a two-stage chromogenic assay procedure for measurement of emicizumab plasma levels

PONE-D-22-10383R1

Dear Dr. Hamedani,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Arijit Biswas

Academic Editor

PLOS ONE