PONE-D-21-16430The effects of compression load to the trunk on lipid metabolism in an inactive phase.PLOS ONE

Dear Dr. Kamei,

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: I Don't Know

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: This study about the compression loading on the murine trunk and related metabolism changes is interesting. This topic is worth to do more study to present more solid information for the understanding and evaluting of this aspect. And, the writing of this manuscript is standard and suitable for reading.

There're questions about the study shown below.

About the concept and the design.

Refer to the metabolism issues, it's well known that the processing is a various long term effects accumulated outcome. In this study, the compression loading lasted only for one day or eight hours. Are there any limitations restricted a long term loading on the animals? Even a 1 week continuous or intermittent treatment could be more convincing about a metabolic topic.

In addition, since it's a study about metabolism as highlighted in the title of this draft, it will be nice if any kind of metabolism related animal models could be utilized, more direct information could be collected for analyzing then.

About the term, animal ethics, and statistics

Please make sure the term concept is correct.

MSCs are not only stem cell of bone cells, and they are not real stem cells according to the definition of stem cell.

“Mice were anesthetized using 2.5% isoflurane at a flow rate of 1 L/min, and the liver samples were collected.” Since there's no euthenizing steps involved, do you mean the tissues collected while the animals are just been anesthetized, still alive?

How many samples used in the transcriptome analysis of liver? There's no N number shown.

Reviewer #2: The manuscript "The effects of compression load to the trunk on lipid metabolism in an inactive phase" investigated the effects of compression load to the trunk on mice metabolism. The findings were interesting, and provided useful information about compression load on health.

1. The authors should discuss why such a short time compression load have the remarkable effects on lipid metabolism in liver, and if a similar effects could be expected on a chronic compression load.

2. The animal numbers used in each experiments should be mentioned in the Method section.

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Reviewer #1: No

Reviewer #2: No

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The effects of compression load to the trunk on lipid metabolism in an inactive phase.

PONE-D-21-16430R1

Dear Dr. Kamei,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Hualin Wang

Guest Editor

PLOS ONE

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