PONE-D-21-40541Melanin Pigment of Streptomyces puniceus RHPR9 Exhibits Antibacterial, Antioxidant, and Anticancer ActivitiesPLOS ONE

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PLOS ONE

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Additional Editor Comments:

For the Manuscript entitled as, Melanin Pigment of Streptomyces puniceus RHPR9 Exhibits Antibacterial, Antioxidant,and Anticancer Activities, please look carefully into the comments and do the changes

Moreover, this paper needs a comprehensive re check for language issues and grammatical mistakes

Add DMRT in Fig 3 and Fig 4B

Improve material n method section and add recent relevant references in discussion

[Note: HTML markup is below. Please do not edit.]

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: • There are typographical errors throughout the MS and authors need to check on English

• There is no recent literature cited related to the work done and needs to be added

• The authors have to correlate the importance of antibacterial and anti-oxidant activity done

• Why only DPPH assay was used for anti-oxidant activity in this study

• Table 3.4.1, is not explained clearly, the title and the subheading needs to be mentioned accordingly with the

melanin pigment used and the antibacterial activity measured

• In M and M, line 69, the details of information about the identification can be cited

• The genus and species need to be italicize throughout the MS for Scientific names

• Figure 1A,1B, 1C can be given as supplementary data

• The authors need to explain clearly how the characterization of pigment is reported as Eumelanin

• Discussion needs to be improved with latest literature, with importance of melanin

Reviewer #2: Authors have reported on Production, characterization of melanin pigment from Streptomyces puniceus RHPR9 and its antibacterial, antioxidant and anticancer activities. Streptomyces spp. from medicinal plant have been explored for various applications. It is a good piece of work and well written, however, the following corrections can be made.

• Line no 149 The word Invitro has to be italicised.

• Line 185 Rewrite the sentence as Purified melanin has pure black colour, which is characteristic among all melanins instead of Purified melanin has a pure black colour, which is characteristic among all melanins.

• Section 2.3 can be modified. Authors can remove the lines that do not describe methodology.

• Section 3.4.1 Table 1 Rewrite the heading and subheadings of the table accordingly. (Mention the concentration of melanin separately).

• Authors can change the labelling of Figure 1C.

• No images of the antibacterial activity have been provided by the authors. Authors can include the images either in the manuscript or as a supplementary source

• Authors can improvise the discussion by correlating antibacterial and antioxidant activity.

• Typographical errors in the manuscript have to be rechecked.

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Reviewer #1: No

Reviewer #2: Yes: Sonia Seifi

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Melanin Pigment of Streptomyces puniceus RHPR9 Exhibits Antibacterial, Antioxidant, and Anticancer Activities

PONE-D-21-40541R1

Dear Dr. Hameeda

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Noshin Ilyas

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Nil

Reviewers' comments:

NIl