PONE-D-21-18861

Interleukin-17 is disease promoting in early stages and protective in late stages of experimental periodontitis

PLOS ONE

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We look forward to receiving your revised manuscript.

Kind regards,

Dr. Kunaal Dhingra, MDS, MFDS RCPS (Glasg), MFDS RCS (Eng), MDTFEd

Academic Editor

PLOS ONE

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This work was supported by the German–Israeli Foundation for Scientific Research and Development Grant 1432 (to A.-H.H. and I.P.), by a grant from the Niedersächsisch-Israelische Forschungsförderung (to A.-H.H. and I.P.), and by the DG PARO CP GABA (to A.W., I.P., and M.S.) A. Wilharm and C. Binz were supported by the Hannover Biomedical Research School.

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Please remove any funding-related text from the manuscript and let us know how you would like to update your Funding Statement. Currently, your Funding Statement reads as follows: 

This work was supported by the German–Israeli Foundation for Scientific Research and Development Grant 1432 (to A.-H.H. and I.P., http://www.gif.org.il/pages/default.aspx), by a grant from the Niedersächsisch-Israelische Forschungsförderung (to A.-H.H. and I.P.), and by the DG PARO CP GABA (to A.W., I.P., and M.S.) A. Wilharm and C. Binz were supported by the Hannover Biomedical Research School. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Additional Editor Comments:

The manuscript is well written. Kindly address following concerns by reviewers:

Introduction length should be a little shorter while Discussion longer with details given in Introduction.

The last sentence of the "Discussion" element (Conclusion): "in the end IL-17 has a protective function for the organism, but a damaging for the bone" seems too general.

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: As the role of IL-17 within the dental tissues is still insufficiently clarified, this study brings very interesting findings in order to provide explanation for dual effects of IL-17. This research is well designed, conducted and presented. Minor suggestion is the Introduction length (should be a little shorter), while Discussion longer with details given in Introduction.

Reviewer #2: The manuscript entitled "Interleukin-17 is disease promoting in early stages and protective in late stages of experimental periodontitis" is nicely prepared. Though, the last sentence of the "Discussion" element (Conclusion): "in the end IL-17 has a protective function for the organism, but a damaging for the bone" seems too general.

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Reviewer #1: No

Reviewer #2: No

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PONE-D-21-18861R1Interleukin-17 is disease promoting in early stages and protective in late stages of experimental periodontitisPLOS ONE

Dear Dr. Binz,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Please submit your revised manuscript by Mar 10 2022 11:59PM. If you will need more time than this to complete your revisions, please reply to this message or contact the journal office at plosone@plos.org. When you're ready to submit your revision, log on to https://www.editorialmanager.com/pone/ and select the 'Submissions Needing Revision' folder to locate your manuscript file.

Please include the following items when submitting your revised manuscript:

• A rebuttal letter that responds to each point raised by the academic editor and reviewer(s). You should upload this letter as a separate file labeled 'Response to Reviewers'.
• A marked-up copy of your manuscript that highlights changes made to the original version. You should upload this as a separate file labeled 'Revised Manuscript with Track Changes'.
• An unmarked version of your revised paper without tracked changes. You should upload this as a separate file labeled 'Manuscript'.

If you would like to make changes to your financial disclosure, please include your updated statement in your cover letter. Guidelines for resubmitting your figure files are available below the reviewer comments at the end of this letter.

If applicable, we recommend that you deposit your laboratory protocols in protocols.io to enhance the reproducibility of your results. Protocols.io assigns your protocol its own identifier (DOI) so that it can be cited independently in the future. For instructions see: https://journals.plos.org/plosone/s/submission-guidelines#loc-laboratory-protocols. Additionally, PLOS ONE offers an option for publishing peer-reviewed Lab Protocol articles, which describe protocols hosted on protocols.io. Read more information on sharing protocols at https://plos.org/protocols?utm_medium=editorial-email&utm_source=authorletters&utm_campaign=protocols.

We look forward to receiving your revised manuscript.

Kind regards,

Kunaal Dhingra, MDS, MFDS RCPS (Glasg), MFDS RCS (Eng), MDTFEd

Academic Editor

PLOS ONE

Journal Requirements:

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

Reviewers' comments:

Reviewer #3: I found the manuscript “Interleukin-17 is disease promoting in early stages and protective in late stages of experimental periodontitis” interesting. It is well written and the methodology is rigorous.

I think that the abstract section is not quite informative. In particular lines 22-30 are a sort of “introduction section”, lines 31-33 represent “methods section”, whereas a few lines are dedicated to the results. I suggest to reduce the introductory part and pay more attention for the results obtained. Furthermore, I suggest to better specify in lines 34-36 what is intended for “bone-damaging” and “bone-protective” effects.

The study focuses on the analysis of IL-17. I believe it would be appropriate to implement the introduction and discussion sections by better investigating the role of this cytokine in the human body and in other oral disorders, especially those that are immune-mediated and / or potentially malignant.

Except for these small improvements, the topic of the study is very interesting and the effort of the authors is to be appreciated.

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While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

Interleukin-17 is disease promoting in early stages and protective in late stages of experimental periodontitis

PONE-D-21-18861R2

Dear Dr. Binz,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Kunaal Dhingra, MDS, MFDS RCPS (Glasg), MFDS RCS (Eng), MDTFEd

Academic Editor

PLOS ONE

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

I thank the authors for making the required changes. The manuscript can be accepted for publication.