PONE-D-21-33082“Isolation, identification and whole genome sequencing of high-yield protease bacteria from daqu of Zhanggong Laojiu”PLOS ONE

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: Check the Reference format. Some references are unavailable with the title search. The findings are interesting. Enough data has been represented to support the article. Overall, this is a nice article!

Reviewer #2: The manuscript is interesting and write up is good. However, the significance of identifying, screening and whole genome sequencing of high-yield protease bacteria are not clear. The author should add some sentences to justify their research.

Some other corrections are listed below.

Line 16: Correct spacing problem as U/mL (Abstract)

Line 43: Bacillus should be Italic (Introduction, 2nd paragraph)

-No need to write Bacillus in every cases. Write the genus name first and then write only the short form like B. amyloliquefaciens, B. subtilis etc throughout the manuscript (Line 72, 75)

-Figure caption (Fig.4 or Fig 5 etc) is not similar. Please follow the exact rules of writing.

-Is it necessary to discuss what other did in their experiment in the conclusion sections? My recommendation is to transfer them in the discussion section. I surprised there is no discussion section. Please add discussion sections and discuss based on the previous findings. Highlighted the significant findings, suggestions, further research possibilities in conclusion section.

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: identification and whole genome sequencing of bacteria.docx

“Isolation, identification and whole genome sequencing of high-yield protease bacteria from daqu of Zhanggong Laojiu”

PONE-D-21-33082R1

Dear Dr. Yanbo Liu,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Tofazzal Islam, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: