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PONE-D-21-2424517R/S-Benzo-RvD1, a synthetic resolvin D1 analogue, attenuates neointimal hyperplasia in a rat model of acute vascular injuryPLOS ONE

Dear Dr. Conte,

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PLOS ONE

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MSC - co-Founder of VasaRx and co-inventor of IP related to this work with the Regents of University of California and Brigham and Women's Hospital”

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Reviewers' comments:

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Comments to the Author

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The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: The paper proposed by Kim AS et al is titled : « 17R/S-Benzo-RvD1, a synthetic resolvin D1 analog, attenuates neointimal hyperplasia in a rat model of acute vascular injury ».

The authors demonstrated that neointimal hyperplasia (NIH) is attenuated by RvD1 treatment following acute arterial injury in rats.

The authors adopted an interesting translational approach to describe their message.

They used VSMC, endothelial cells, HUVEC, and an in vivo model of induced intimal hyperplasia in rats.

Their multidisciplinary methods include migration assay, cell shape measurement, cytotoxicity, cell proliferation, immunostaining, in vivo analyses.

Although the paper is well designed and written, some minor concerns need to be addressed before acceptance.

1- Add a reference for the two first sentences of the introduction.

2- In the statistical analysis paragraph of the Material and methods section, add information about the minimum p from which data were considered significant. For example, p<0.05.

3. In multiple group analyses, one way ANOVA followed by Bonferroni or Tuckey post-hoc tests may better apply in this context. The authors can either repeat the appropriate statistics tests when applicable, or justify their choice in a limitation section.

4. As the `n` are low, for transparency, the authors are invited to present all bar graphs as scatter/dot plot graphs, showing mean’s and SEM’s bars. (except graphs shown in supplementary figures, as scatter/dot plot may be less readable than bar graphs).

5. Figure 3 and 4, add a scale for the pictures.

6. Add a limitation section after the discussion and before the conclusion to address the main challenges met by this study, the limitations, and possible ways to overcome them in future investigations. This can include justification of the small number of samples n = 4 or 3; feasibility of further experiments or analyses (qPCR, western blot, etc).

7. Add a Highlight section with up to 5 bullet points to describe the main findings of the paper or/and patient-oriented potential applications.

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Reviewer #1: Yes: Roddy Hiram

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17R/S-Benzo-RvD1, a synthetic resolvin D1 analogue, attenuates neointimal hyperplasia in a rat model of acute vascular injury

PONE-D-21-24245R1

Dear Dr. Conte,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Michael Bader

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: