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PONE-D-21-21932Parous rate and longevity of anophelines mosquitoes in Bure district, northwestern EthiopiaPLOS ONE

Dear Dr. Adugna,

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'A collection of mosquitoes was carried out after obtaining ethical approval

from the ethics review committee of Addis Ababa University (reference no.

CNSDO/382/07/15), Amhara Health Regional Bureau (permission reference no.

H/M/TS/1/350/07) and the Head of the Bure District Health Office (permission

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Additional Editor Comments (if provided):

I like this paper, you have written it well and it should be published. My concern, and I would like you to rewrite this part. This deals with the introduction and the discussion of parous rates, Parity or non-parous are two conditions. A parous mosquito could have laid one or more batches of eggs. Th only way to expand parity is via Detinova's technique. But it is very difficult to undertake and be accurate. I know I have done it. You quote from ref 27about the length of a gonotrophic cycle, i.e. the time passed between laying egg batches. Really this can only be done in caged mosquitoes, but as they are poikilothermic, the ambient temperature is important. I have read ref 27, it is somewhat hypothetical, and I am not sure it is feasible to compare with nature.

We all have tried to age the life span of female mosquitoes, but the answer is frustratingly difficult.

Please also review the sentence "Usually, older females also have higher exposure rates... it is unclear. please do not suggest that we can judge the life span of a mosquito by parous rates, because we cannot estimate how many ovipositions had taken place. please also note that Davidson's work on longevity has been disputed, (see ref 27)

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Parous rate and longevity of anophelines mosquitoes in Bure district, northwestern Ethiopia

PONE-D-21-21932R1

Dear Dr. Adugna,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Reviewers' comments: