PONE-D-21-22141

A pattern of Platelet Indices as a Potential Marker for Prediction of Pre-eclampsia among Pregnant Women Attending Wachemo University Nigist Eleni Mohammed Memorial Comprehensive and Specialized Hospital, Southern Ethiopia

PLOS ONE

Dear Dr. Bawore,

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PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: No

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Title: A pattern of Platelet Indices as a Potential Marker for Prediction of Pre-eclampsia among Pregnant Women Attending Wachemo University Nigist Eleni Mohammed Memorial Comprehensive and Specialized Hospital, Southern Ethiopia

Comments:

1) Title : Too long , please delete the setting name and replace it for instance (A pattern of Platelet Indices as a Potential Marker for Prediction of Pre-eclampsia among Pregnant Women Attending A tertiary hospital at Ethiopia !!!!!)

2) Abstract:

1. The practice of platelet indices in the diagnosis of preeclampsia is not yet extensive in Ethiopia and there is little information on platelet patterns in preeclampsia and normal pregnancy!!! : it is not an evidence based method to diagnose preeclampsia using platelet indices for most of obstetric settings and of course including Ethiopia .

Please rephrase this statement

2. The purpose of this study was to assess the pattern of platelet indices for the prediction of preeclampsia in pregnant: Prediction means you have to follow a large number of pregnant women whom you suspect to have PE in the future time, then to assess their platelet indices frequently until you have reached a level that can give you a clue that she has developed PE !!! . This is not what you have done! You have already diagnosed women having PE and their platelet indices was assessed and showed a difference in comparison to the normal group.

This mostly follows the severity of PE not as a diagnosis or prediction for future time .

My suggestion is to change your aim to : The purpose of this study was to assess the pattern of platelet indices in women with preeclampsia in your setting

3) Patients and Methods :

1. You need to define cases of preeclampsia, mild and sever eclampsia in a separate sub section giving the reference at the end. Also definitions for variables used in socieo demographic characters for instance BMI!!!!

2. What was your reference to identify all the platelet indices as normal? Which reference you have used to be abnormal? And what do you meant by platelet indices?

3. How many times you have estimated the platelet indices? I assume once!!!you have to record it in the methods section

4) Result section:

1. It seems that your design of the study is not a cross sectional study!!!! it is a case control study , you have identified your criteria to involve cases and controls before starting .Please change it in the whole text to a case control study .Do not use a comparative !!!

2. Again define how did you categorized your cases to sever and mild or severer and non-sever? adding a reference to them and make it the same words in the text and in the tables

3. Add a footnotes for all the tables including the full name of the abbreviations

Reviewer #2: This manuscript is sound in experimental design and draws reasonable conclusions from the data collected. This work provides novel insight into the mechanisms that may advance detection of preclampsia. However, there are consistent grammatical errors that compromise understanding of the methodology and findings. It is my opinion that this manuscript needs to be copyedited and reviewed to ensure that those edits retain the original meaning before full acceptance.

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Reviewer #1: Yes: Shahla Alalaf

Reviewer #2: No

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A Pattern Of Platelet Indices As A Potential Marker For Prediction Of Pre-Eclampsia Among Pregnant Women Attending A Tertiary Hospital , Ethiopia: A Case-Control Study

PONE-D-21-22141R1

Dear Dr. Bawore,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Linglin Xie

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: (No Response)

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: Yes: Shahla Alalaf

Reviewer #2: No