PONE-D-21-22172Design, Synthesis and Biological Evaluation of N-oxide Derivatives with Potent In Vivo Antileishmanial ActivityPLOS ONE

Dear Dr. Graminha,

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PLOS ONE

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“This study was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP Process: 2016/09502-7, 2016/06931-4, 2018/17739-2, 2018/11079-0 and 2017/03552-5). Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP – PADC. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). L.C.C. is CNPq PhD student fellow (143334/2017-4). I.M.P is CNPq postdoctoral fellow (153581/2018-2). J.L.S, F.H.S. and M.A.S.G are recipient of a Research Productivity Scholarship from the National Council for Research and Development (CNPq Ref. Process: 304731/2017-0, 311746/2017-9 and 305174/2020-7, respectively). The authors are grateful to Profº André Tempone and Felipe Torres for Leishmania infantum (MHOM/MA67IMA263) donation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.”

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Additional Editor Comments (if provided):

The manuscript reports synthesis of 24 compounds which are tested on Leishmania-infected BALB/c mice for their anti-leishmanial activities. The identified one compound show significant anti-leishmanial activity in both spleen and liver.

However, the manuscript requires several moderations as follows:

1. Please merge Figures 2, 3 and 4 into one - Figure 2

2. Please merge Figures 5 and 6 into one - Figure 3

3. Please merge Figure 7 and 8 into one - Figure 4

4. Please move Figure 9 into supplementary figure.

This will help shorten the manuscript. Please check the composition of the manuscript.

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Reviewers' comments:

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Comments to the Author

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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5. Review Comments to the Author

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Reviewer #1: Minor Revision

1. Page 24, Line 566, the figure legend needs to be written more elaborately.

2. Figure Number 5 is unreadable.

3. Page no 28, line number 601, figure number 6B and 6C is cited. No figure 6B and 6C is in the paper.

4. Page no 28, line no 607, figure 7 is cited, the description does not match with figure7.

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Reviewer #1: No

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Design, synthesis and biological evaluation of N-oxide derivatives with potent in vivo antileishmanial activity

PONE-D-21-22172R1

Dear Dr. Graminha,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Bhaskar Saha

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: