Detecting Phytophthora cinnamomi associated with dieback disease on Carya cathayensis using loop-mediated isothermal amplification

PONE-D-21-28959

Dear Dr. Wang,

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Kind regards,

Sagheer Atta, Ph.D

Academic Editor

PLOS ONE

Journal requirements:

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 [The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.]

a) Please provide an amended Funding Statement that declares *all* the funding or sources of support received during this specific study (whether external or internal to your organization) as detailed online in our guide for authors at http://journals.plos.org/plosone/s/submit-now.  

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The research topic/work taken is based on the importance, right now the pathogen is wide host range, moreover it has to be early diagnosed. Experiments were planned very well. The conclusion match with the output of the research work.

Statistical analysis does not need for the present research work. Data required are included in the current research work. Manuscript presented in an intelligible fashion and written in standard English.

Reviewer #2: the manuscript was presented in an intelligible fashion and written in standard English

the statistical analysis has been performed appropriately and rigorously

the manuscript technically is sound, and do the data support the conclusions

the manuscript can be accepted in plos one.

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Reviewer #1: Yes: A Ramanathan

Reviewer #2: Yes: Dr. Sakineh Abbasi