PONE-D-21-27320Two Fingerprinting Sets for Humulus lupulus Based on KASP and Microsatellite MarkersPLOS ONE

Dear Dr. Bassil,

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Vikas Sharma, Ph.D

Academic Editor

PLOS ONE

Additional Editor Comments (if provided):

This research work provided important sets of KASP and SSR markers for Humulus lupulus which will be useful for its characterization and authentication in future works. Overall, manuscript is well written and every related aspect has been well documented and presented. I strongly recommend its publication in PLOS ONE after careful revision to remove lingual errors etc. (line no. 191, line 207: OR can be written in full, line no. 232: using used, line no. 269: sentence starting from sNMF) please correct all such minor issues throughout the manuscript before publication.

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“We thank USDA CRIS 2072-21000-049-00D and CRIS 2072-21000-051-00D for financial support for this project. We thank the Brewers Association for additional funding of this project.”

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: The manuscript is in good shape and properly crafted. The problem and objectives are identified in a systematic way. The methodology of the experiment is scientific, classified and well designed. The dataset and sample pool is reasonably justifiable. The manuscript is written in good, lucid, simple and grammatically correct language. Statistical tools used for the analysis are robust and latest with updated references. The manuscript is recommended for the publications after checking the requirements and formats of the journal.

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Two Fingerprinting Sets for Humulus lupulus Based on KASP and Microsatellite Markers

PONE-D-21-27320R1

Dear Dr. Bassil,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Vikas Sharma, Ph.D

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Authors have revised the manuscript as per minor comments raised previously. Therefore, it can be accepted now. However, authors are advised to revise manuscript to remove any minor errors (lingual or any other) as afterwards no changes will be possible in the manuscript.

Reviewers' comments: