PONE-D-21-12616

Fucosyltransferase 2 inhibitors: Identification of new leads via docking and STD-NMR studies

PLOS ONE

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Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: Humaira Zafar et al submitted an article having the title “Fucosyltransferase 2 inhibitors: Identification of new leads via docking and STD-NMR studies” to on PLOS ONE. This article explains to identify the inhibitors for Fucosyltransferase 2 via docking and STD-NMR studies. Thus I recommended the artcle for the publication with minor revision. The following points need to be noted and revised prior to publication,

1. The authors are suggested to concise the introduction part.

2. Authors should also provide no. of interactions during docking.

3. Article needs to improve quality of the figures.

4. The authors are suggested to give the recent references in support of this work.

Reviewer #2: Response: The manuscript by H. Zafar and coworkers beautifully designed different complexes to identify the inhibitors for FUT2 and studied the cancer properties of these complexes.

The work seems to be carried out thoroughly and the compounds were characterized using various techniques. In silico-screening of natural and synthetic compounds have been performed to better understand the system. Molecular docking studies and epitope mapping in ligands further studied to better explain the interactions of ligands with amino acid residues of the active site of FUT2 and receptor protein.

The manuscript is nicely written and reads well. The introduction starts with fucosyltransferases and ends to form different complexes and their use as therapeutic agents in Cancer. Further, the drug-like properties are confirmed using swiss ADME and ToxiM analysis.I would like to suggest the Author to give the clear NMR for all the complexes, although it is already confirmed using other studies/techniques.

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Reviewer #1: No

Reviewer #2: No

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Fucosyltransferase 2 inhibitors: Identification via docking and STD-NMR studies

PONE-D-21-12616R1

Dear Dr. Zafar,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Mohammad Shahid, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: