PONE-D-21-11819

Validation of conventional PCR-like alternative to SARS-CoV-2 detection with target nucleocapsid protein gene in naso-oropharyngeal samples

PLOS ONE

Dear Dr. Carvalho,

Thank you for submitting your manuscript to PLOS ONE. After careful consideration, we feel that it has merit but does not fully meet PLOS ONE’s publication criteria as it currently stands. Therefore, we invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

Specifically, please summarize the NC functions and the immune responses in natural infections. Relevant references on the viral nucleocapsid N protein are from Luis Enjuanes et al. showing that N is a multifunctional protein with RNA binding and chaperoning activities. In addition specific IGG against N are found in people who experienced Cov infection.

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Jean-Luc EPH Darlix, MG, Ph.D.

Academic Editor

PLOS ONE

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: This is an interesting paper that describes an alternative, PCR-based method for COVID-19 diagnosis, beyond the predominant real-time PCR methods that are being utilized worldwide. While the total amount of data presented and overall scope of the work is limited, the main point of the paper is important and timely. Thus, while this manuscript is much shorter than most full-length articles, I recommend publishing, due to the importance of the findings.

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Reviewer #1: No

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Validation of conventional PCR-like alternative to SARS-CoV-2 detection with target nucleocapsid protein gene in naso-oropharyngeal samples

PONE-D-21-11819R1

Dear Dr. Carvalho,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Jean-Luc EPH Darlix, MG, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: