PONE-D-21-27759Tolerance of Pseudomonas Strain to the 2,4-D Herbicide through a Peroxidase SystemPLOS ONE

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Academic Editor

PLOS ONE

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Additional Editor Comments:

The manuscript 'Tolerance of Pseudomonas Strain to the 2,4-D Herbicide through a Peroxidase System' has been reviewed by two experts and both the reviewers raised concerns and asked the authors submit a revised version for re-consideration. Please revise the MS and provide a point-by-point response to each comment with the revised submission.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: No

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Reviewer Response

The original manuscript titled ‘Tolerance of Pseudomonas Strain to the 2,4-D Herbicide through a Peroxidase System’, manuscript number ‘PONE-D-21-27759’ is an excellent research work and written in a good manner. The manuscript can be considered for publication process after meeting out the essential inclusions and improvements in the details provided. Hence, the authors are kindly requested to do the following,

1. Abstract section needs to modified and rewritten even more effectively avoiding few unnecessary explanations.

2. Provide better keywords in maximum numbers as per journal guidelines.

3. The introduction section can be presented with information of harmful effect of 2,4-D on human health, and can be improved with good research statement.

4. The results and discussion section needs to be modified. The authors can concentrate in providing more supporting discussion for the results obtained in the study. The discussion part is insufficient for understanding the results.

5. Give details on the mechanism of how Pseudomonas Strain managed to tolerate 2,4-D herbicide using Peroxidase System with your experiments.

6. The manuscript has detailed the results captured, but the effective explanations of the research results are missing; kindly update it under each respective header.

7. Conclusion section can be made even better and reachable with your obtained results.

8. The references are needed to be concentrated and aligned properly.

9. The entire alignment of manuscript content is inappropriate, it needs to be corrected.

10. Kindly correct the tenses of language used under each section and also correct the spellings.

Reviewer #2: The manuscript needs following amendments;

Line#122: DNA polymerase is always written in Units like 0.5Units.

Line#123: Kindly, mention the total reaction mixture volume.

Line#124/125: Kindly, mention thermocycler cycles with stages like Initial Denaturation at 95℃/2mins, for 40 cycles: Denaturation at 95℃/30 sec, Annealing at 57.7℃/30sec, Extension at 72℃/3min; Final-Extension at 72℃/5mins & 4℃/∞.

Line#145: Kindly, repeat the above-mentioned suggestion for this section also.

Line#212: MDA stands for?

Line#279/298/315/340/372/412/443/457: As per Journal’s Format, Figure Legends are to be mentioned at end of the text.

Line#279: Kindly, correct the figure legend font as per Journal’s Format.

Line#392: Kindly, correct “as ah response system”, as “as response system”.

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Reviewer #1: No

Reviewer #2: Yes: Syeda Zahra Abbas

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Attachments

Attachment

Submitted filename: Tolerance of Pseudomonas Strain to the 2,4-D Herbicide plos one.doc

Attachment

Submitted filename: Manuscript Number PONE-D-21-27759.docx

Tolerance of Pseudomonas Strain to the 2,4-D Herbicide through a Peroxidase System

PONE-D-21-27759R1

Dear Dr. Marcos Pileggi

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Muhammad Shahid, PhD

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: