PONE-D-21-10171

Phylogeography of Baryancistrus xanthellus (Siluriformes: Loricariidae), a rheophilic catfish endemic to the Xingu River basin in eastern Amazonia

PLOS ONE

Dear Dr. Sousa,

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Both reviewers had relatively few comments and suggestions and they agree that the manuscipt should be published in this journal. 

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Juan Marcos Mirande

Academic Editor

PLOS ONE

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Reviewers' comments:

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Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: N/A

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Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript deals with a variable catfish species in a proper way, analyzing genetically different subpopulations and concluding about their phylogeographic history. This study was long needed and is presently beautifully. The manuscript is generally well researched and well written, and include very good, highly illustrative figures.

I only have a few corrections before acceptance, which are listed below, by line number:

Lines 7, 9, 10, 12 and 14: Please add the postal code (CEP) to all Brazilian addresses before the city name, and without a separating comma.

Line 51: It is numerous but obviously countable… so countless is not a good adjective. Replace by numerous or other similar word.]

Line 57: Those three basins .. drain the …

Line 58: ... their downstream portions lie on the …

61: Xingu River

81: color (stick to American English)

104: middle Xingu River (middle is not part of the name)

109: Xingu River basin (basin is not part of the name)

117 and 246: I think “ria” is not an English word. It is usually translated as estuary, despite a “ria” opens to another river. Perhaps you should explain the meaning of “ria” at first usage in M&M, or chose a different way of saying ria.

119: middle Xingu

314: Demographic

347: xanthellus

351: Critically Endangered, without quotes and with capital initials (as recommended by IUCN).

419: add space after rapids,

454: responded

463-4: over the entire species geographic range.

Congratulations on the very good figures!

R. E. Reis

Reviewer #2: Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters) (Limit 200 to 20000 Characters)

Could you please see my attached review, I have added all my comments to the Author in an attachment.

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Reviewer #1: No

Reviewer #2: No

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Attachments

Attachment

Submitted filename: rev_plos_Bxant.pdf

PONE-D-21-10171R1

Phylogeography of Baryancistrus xanthellus (Siluriformes: Loricariidae), a rheophilic catfish endemic to the Xingu River basin in eastern Amazonia

PLOS ONE

Dear Dr. Sousa,

All the suggestions from the reviewers were followed and I find the manuscript ready to be published.

I just found one word that went back to British English in the latest review, that should be kept in American English to be congruent. In Line 114, please change back "Analysed" to "Analyzed". As the journal hasn't copy-editing, it should be done by the authors.

After that, the manuscript will be sent to production.

Sincerely, Marcos Mirande

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Reviewers' comments:

[NOTE: If reviewer comments were submitted as an attachment file, they will be attached to this email and accessible via the submission site. Please log into your account, locate the manuscript record, and check for the action link "View Attachments". If this link does not appear, there are no attachment files.]

While revising your submission, please upload your figure files to the Preflight Analysis and Conversion Engine (PACE) digital diagnostic tool, https://pacev2.apexcovantage.com/. PACE helps ensure that figures meet PLOS requirements. To use PACE, you must first register as a user. Registration is free. Then, login and navigate to the UPLOAD tab, where you will find detailed instructions on how to use the tool. If you encounter any issues or have any questions when using PACE, please email PLOS at figures@plos.org. Please note that Supporting Information files do not need this step.

Phylogeography of Baryancistrus xanthellus (Siluriformes: Loricariidae), a rheophilic catfish endemic to the Xingu River basin in eastern Amazonia

PONE-D-21-10171R2

Dear Dr. Sousa,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Juan Marcos Mirande

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments: