PONE-D-21-18913

Differential mitochondrial protein interaction profile between human translocator protein and its A147T polymorphism variant

PLOS ONE

Dear Dr. Ittner,

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Anjani Kumar Tiwari, Ph.D.

Academic Editor

PLOS ONE

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This works was supported by funding from the National Health and Medical Research Council 

13 and the Australian Research Council to M.K., Y.D.K. and L.M.I..

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Additional Editor Comments:

This is very good attempt to see the interactions, but results are not very promising. Further supporting data are required . Author may give insights of additional studies if possible and should add a detailed future studies required in this directions. It will help to those who are trying to establish new insights for this protein in different pathological conditions.

Please review your reference list to ensure that it is complete and correct. If you have cited papers that have been retracted, please include the rationale for doing so in the manuscript text, or remove these references and replace them with relevant current references. Any changes to the reference list should be mentioned in the rebuttal letter that accompanies your revised manuscript. If you need to cite a retracted article, indicate the article’s retracted status in the References list and also include a citation and full reference for the retraction notice.

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: No

Reviewer #2: Yes

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: No

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: The manuscript of the paper entitled "Differential mitochondrial protein interaction profile between human translocator protein and its A147T polymorphism variant' is presented in a proper manner. However, author should consider following corrections before resubmission:

1.Page 10, L16 replace ‘cOmplete with ‘complete’

2.Page 10, L17; Page 10, L 22; Page 11, L 6, replace ‘))’ with ‘)’

3.Page 11, L 13 replace ‘inhibitor .’ with ‘inhibitor.’

4.Maintain consistency in units such as 50 mM instead of 50mM, 4oC instead of 4o C, min instead of Minutes, h instead of hour, either mL or ml, g instead of gram, 10 cm instead of 10cm, μl instead of microlitres etc

5.Page 12, L 13-15, L16-18 correct the sentences

6.Page 17, L12-14, reframe the sentence

7.Page 20, line 8, Replace ‘experiments .’ with ’experiments.’

I found the experiments were executed in a planned way. Technically manuscript is good.

Reviewer #2: An interesting research work by Lars M Ittner and team.

This is first such study to see interaction profile between human translocator protein and its A147T polymorphism variant.

In summary, the results identified the loss of a subset of interaction partners with the 6 A147T polymorphism variant of human TSPO but one of the positive aspects seems

"TSPO’s interaction with YWHAQ 7 might contribute to the regulation of TSPO’s targeting into mitochondrial membranes involved 8 in apoptotic signaling pathways"

Does author further support it by additional evidence.

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Reviewer #1: Yes: Dr Pooja Srivastava

Reviewer #2: No

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Differential mitochondrial protein interaction profile between human translocator protein and its A147T polymorphism variant

PONE-D-21-18913R1

Dear Author

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Anjani Kumar Tiwari, Ph.D.

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

This form of manuscript is ok for publication.

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

Reviewer #2: All comments have been addressed

Reviewer #3: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #2: Yes

Reviewer #3: Yes

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3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #2: N/A

Reviewer #3: Yes

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4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #2: Yes

Reviewer #3: No

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5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #2: No

Reviewer #3: Yes

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6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #2: As i can see that nearly all the questions asked by experts have been replied in revised manuscript therefore in my opinion Academic Editor may permit for publication in PLOS ONE

Reviewer #3: All the queries raised by previous reviewers have been replied, therefore it can be considered for publication in PLOS One.

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #2: No

Reviewer #3: No