Laminar flow substantially affects the morphology and functional phenotype of glomerular endothelial cells

PONE-D-21-07368

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Reviewer #1: Yes

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

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Reviewer #1: In their very well designed comprehensive study the Authors present novel findings on the effects of a long-term shear stress on glomerular endothelial cells in culture. The Authors have investigated and characterized the key GEnC features that are typically sensitive to the shear stress, such as NO synthesis, expression of stress-responsive genes and proteins, or cytoskeleton shape.

Presented results are convincing and they clearly indicate that cells in culture should be exposed to stimuli similar to those in vivo. Otherwise, the results may not sufficiently reflect the actual cell reaction.

On the other hand, this is a very inspiring study that should be (and I believe, will be) followed by next experiments investigating the reciprocal contacts between the GEn cells and the podocytes , in conditions where both cell types are grown under laminar flow.

Reviewer #2: In my view, it is a flawless study. No suggestions for improvements

This is a well-done study on an important subject. Such a study has been overdue. I recommend to accept this manuscript without any changes. In my view, it is a flawless study.

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Reviewer #1: Yes: Barbara Lewko, PhD, Department of Pharmacological Pathophysiology, Faculty of Pharmacy, Medical University of Gdansk, Poland

Reviewer #2: No