PONE-D-20-37779

Copaiba essential oil (Copaifera spp.) as natural preservative on sheep burgers: shelf life and consumer acceptability

PLOS ONE

Dear Dr. Monteschio,

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Academic Editor

PLOS ONE

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Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Partly

Reviewer #2: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: No

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3. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

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4. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

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5. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: MS : PONE-D-20-37779

COMMENTS:

1. Title needs to be changed into a continuous title, remove acceptability as it is understood that organoleptic properties are assessed when studying a food preservative

2. Abstract....... . Assessing during it shelf life the antioxidant activity, lipid oxidation, pH, color, cooking loss, texture and consumer acceptability. Rewrite this line ...

3. Mention species of Copaiba used for extraction of essential oil........this is very important as essential oil component changes with species...it should also reflect in title for the sake of future readers.

4. Material and methods

� Give details of collection of plants or procurement of oil. If oil was extracted the give procedure and storage details...get plant species identified

� Give at least minor procedural details of GC_MS and library used

� Mention table 1 in GCMS section

� How oil was used for treatment is not clear .please write it clearly

� In result section the tables and figures should be arranged in sequential pattern. At several places there is incoherence leading to confusion.....

5. Discussion should be more critical citing other similar works on same commodity. As far as antioxidant property of oil is concerned almost all EO have it and this discovery is not new unless it is correlated to a specific species which is lacking.

6. Evolution of.....why Evolution ...i cannot understand...use some other term

7. Conclusion is just repetition of abstract....write a critical conclusion

8. Recheck the references and numbering

Reviewer #2: 1. The authors should describes more details about the experimental design particularly the concentrations tested such as BHT and CEO. In CEO treatments, why use 0.1 and 0.05 but not other concentrations?

2. Proper statistical analysis should be applied to the concentrations in Table 1.

3. Introduction section: Adds more contents about the practical use and literature of essential oils in the food industry. Adds a paragraph describing the constituents and efficacy of Copaiba essential oil.

4. Abstract: as possible replacement - as a possible replacement; Assessing during it shelf life - Assessing during its shelf life; essential oil showed antioxidant effect - essential oil showed an antioxidant effect; the fresh‐ like quality - the fresh‐like quality

5. Introduction: evaluating it influence - evaluating its influence

6. A representative chromatography spectra for chemical constituents of Copaiba essential oil is needed.

7. The authors should check grammar errors throughout the manuscript and improve them.

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6. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No

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Effect of copaíba essential oil (Copaifera officinalis L.) as a natural preservative on the oxidation and shelf life of sheep burgers

PONE-D-20-37779R1

Dear Dr. Monteschio,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you’ll receive an e-mail detailing the required amendments. When these have been addressed, you’ll receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Christophe Hano

Academic Editor

PLOS ONE

Additional Editor Comments (optional):

Reviewers' comments:

Reviewer's Responses to Questions

Comments to the Author

1. If the authors have adequately addressed your comments raised in a previous round of review and you feel that this manuscript is now acceptable for publication, you may indicate that here to bypass the “Comments to the Author” section, enter your conflict of interest statement in the “Confidential to Editor” section, and submit your "Accept" recommendation.

Reviewer #1: All comments have been addressed

Reviewer #2: All comments have been addressed

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2. Is the manuscript technically sound, and do the data support the conclusions?

The manuscript must describe a technically sound piece of scientific research with data that supports the conclusions. Experiments must have been conducted rigorously, with appropriate controls, replication, and sample sizes. The conclusions must be drawn appropriately based on the data presented.

Reviewer #1: Yes

Reviewer #2: Yes

**********

3. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

Reviewer #2: Yes

**********

4. Have the authors made all data underlying the findings in their manuscript fully available?

The PLOS Data policy requires authors to make all data underlying the findings described in their manuscript fully available without restriction, with rare exception (please refer to the Data Availability Statement in the manuscript PDF file). The data should be provided as part of the manuscript or its supporting information, or deposited to a public repository. For example, in addition to summary statistics, the data points behind means, medians and variance measures should be available. If there are restrictions on publicly sharing data—e.g. participant privacy or use of data from a third party—those must be specified.

Reviewer #1: Yes

Reviewer #2: Yes

**********

5. Is the manuscript presented in an intelligible fashion and written in standard English?

PLOS ONE does not copyedit accepted manuscripts, so the language in submitted articles must be clear, correct, and unambiguous. Any typographical or grammatical errors should be corrected at revision, so please note any specific errors here.

Reviewer #1: Yes

Reviewer #2: Yes

**********

6. Review Comments to the Author

Please use the space provided to explain your answers to the questions above. You may also include additional comments for the author, including concerns about dual publication, research ethics, or publication ethics. (Please upload your review as an attachment if it exceeds 20,000 characters)

Reviewer #1: Accepted with some minor formatting according to Plos One policy. The authors be advised to recheck the language at proof stage. The MS is a nice piece of work with practical acceptability

Reviewer #2: (No Response)

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7. PLOS authors have the option to publish the peer review history of their article (what does this mean?). If published, this will include your full peer review and any attached files.

If you choose “no”, your identity will remain anonymous but your review may still be made public.

Do you want your identity to be public for this peer review? For information about this choice, including consent withdrawal, please see our Privacy Policy.

Reviewer #1: No

Reviewer #2: No