PONE-D-21-01454

Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions

PLOS ONE

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Reviewer #1: Partly

Reviewer #2: Yes

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Reviewer #2: Yes

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Reviewer #1: No

Reviewer #2: Yes

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Reviewer #2: No

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5. Review Comments to the Author

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Reviewer #1: 1: A certain view is implied by the authors regarding to this study but they could be more explicit.

The authors take a rather narrow view of data publication, which I think hinders their analyses. In this study, author need to elaborate further manuscript methodology and results and results should be reconfirmed with other assay as well.

2: Manuscript writeup should improve.

3: Figure legends could not show proper description, So its better to write in detail.

Reviewer #2: In this manuscript entitled Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions analyze six genes (HPRT, ACTB, PGAM-1, GAPDH, PPIA, and B2M) using five software applications and the ΔCt method to determine that the best genes to be used for mesangial cell studies with high glucose and angiotensin receptor II blocker are HPRT and ACTB, while in the same conditions, the best combination of genes for podocyte gene expression normalization is HPRT. The study described in the manuscript is interesting however the manuscript can be considered for publication only if the following issues are addressed:

Major Comments

1 Why authors select these six genes (HPRT, ACTB, PGAM-1, GAPDH, PPIA, and B2M). Please elaborate in this article with references

2 Authors should also compare the results of syber green with probe based real time PCR

3. Grammatical errors in all parts of manuscript

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Reviewer #1: No

Reviewer #2: No

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Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions

PONE-D-21-01454R1

Dear Dr. Boim,

We’re pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

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Kind regards,

Muhammad Shareef Masoud, Ph.D

Academic Editor

PLOS ONE