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PONE-D-20-30737

Minimizing isotropic and deviatoric membrane energy – an unifying formation mechanism of different cellular membrane nanovesicle types

PLOS ONE

Dear Dr. Kralj-Iglič,

Thank you for submitting your manuscript to PLOS ONE. I have completed the evaluation of your manuscript with the comments from the reviewer. As there are few typos in the manuscript, minor revision is required. I invite you to submit a revised version of the manuscript that addresses the points raised during the review process.

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Xin Yi

Academic Editor

PLOS ONE

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Reviewer #1: Yes

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2. Has the statistical analysis been performed appropriately and rigorously?

Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: Yes

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Reviewer #1: The manuscript “Minimizing isotropic and deviatoric membrane energy – an unifying formation mechanism of different cellular membrane nanovesicle types” describes the formation of the nanovesicles within the deviatoric curvature model. The deviatoric curvature model is an extension of the commonly studied spontaneous curvature model. It allows for accounting the shape and orientation of the membrane constituents. Therefore, it gives more freedom in interpretation of the experimental results. The authors try to explain the experimental observation on formation of nanovesicles based on the results of the numerical calculations with a specific set of parameters for the deviatoric curvatures model. The deviatoric model is not so commonly used as the spontaneous curvature model. Such new results may be of interest to the scientific community, and may be helpful in understanding the processes which are encountered in biological membranes. I think that the results presented in the manuscript have sufficient element of novelty to be published in PlosOne.

Minor comment: in eq (7), line 670, there is a typo. There should be (m2/m) instead of (m1/m) in the second lg() of entropy of mixing term

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Reviewer #1: No

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Minimizing isotropic and deviatoric membrane energy – an unifying formation mechanism of different cellular membrane nanovesicle types

PONE-D-20-30737R1

Dear Dr. Veronika Kralj-Iglič,

We are pleased to inform you that your manuscript has been judged scientifically suitable for publication and will be formally accepted for publication once it meets all outstanding technical requirements.

Within one week, you will receive an e-mail detailing the required amendments. When these have been addressed, you will receive a formal acceptance letter and your manuscript will be scheduled for publication.

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Kind regards,

Xin Yi

Academic Editor

PLOS ONE